Small RNA deep sequencing revealed microRNAs? involvement in modulating cellular senescence and immortalization state

文献类型： 外文期刊

第一作者： Zhu, Chen

作者： Zhu, Chen;Zhang, Lei;Heidari, Mohammad;Zhang, Huanmin;Zhu, Chen;Zhang, Lei;Sun, Shuhong;Xie, Qingmei;Ai, Yongxing;Dong, Kunzhe

作者机构：

关键词： spontaneously immortalized cells; microRNAs; microRNA expressions; predicted target genes; pathways

期刊名称：POULTRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）

ISSN： 0032-5791

年卷期： 2023 年 102 卷 3 期

页码：

收录情况： SCI

摘要： Unlike rodent cells, spontaneous immor-talization of avian cells and human cells is a very rare event. According to patent publications and current literature, there are no more than 4 spontaneously immortalized chicken embryo fibroblast (CEF) cell lines established up to date. One of those cell lines is ADOL (Avian Disease and Oncology Laboratory) ZS-1 cell line, which was established by continuous passag-ing of the CEFs derived from the specific pathogen free (SPF) 0.TVB*S1 (commonly known as rapid feather-ing susceptible or RFS) genetic line of chickens. The RFS genetic line of chickens was developed and has been maintained on the SPF chicken farm of USDA-ARS facility, ADOL, in East Lansing, Michigan, which is known as one of a few lines of chickens that are free of any known avian endogenous virus genes. To explore potential roles that epigenetic factors may play in mod-ulating cellular senescence processes and spontaneous immortalization state, total RNAs extracted from sam-ples of the RFS primary CEFs, RFS CEFs reached the 21st passage, and the ZS-1 cells were subjected to small RNA sequencing. Collectively, a total of 531 miRNAs was identified in the 3 types of samples. In contrast to the primary CEF samples, 50 miRNAs were identified with significantly differential expression only in the 21st passage samples; a different subset of 63 differen-tially expressed miRNAs was identified only in the ZS -1 samples; the majority of differentially expressed miR-NAs identified in both the 21st passage CEF and the ZS-1 samples were more or less directionally consistent. Gene Ontology analysis results suggested that the epi-genetic factor, miRNAs, plays a role in modulating the cellular senescence and spontaneous immortalization processes through various bioprocesses and key path-ways including ErbB and MAPK signaling pathways. These findings provided the experimental and bioinfor-matic evidence for a better understanding on the epige-netic factor of miRNAs in association with cellular senescence and spontaneous immortalization process in avian cells.

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