Comparative proteomic analysis of wild-type and a SlETR-3 (Nr) mutant reveals an ethylene-induced physiological regulatory network in fresh-cut tomatoes

文献类型： 外文期刊

第一作者： Shi, Jun-yan

作者： Shi, Jun-yan;Wang, Qian;Zuo, Jin-hua;Zheng, Shu-fang;Gao, Li-pu;Liu, Jia;Wang, Qing;Shi, Jun-yan;Wang, Qian;Zuo, Jin-hua;Zheng, Shu-fang;Gao, Li-pu;Wang, Qing;Wang, Qian;Liu, Jia;Shi, Jun-yan

作者机构：

关键词： Fresh-cut tomato; Proteomics; Ethylene; Pectinesterase; Physiological regulatory network

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：7.425； 五年影响因子：7.716 ）

ISSN： 0963-9969

年卷期： 2022 年 161 卷

页码：

收录情况： SCI

摘要： Ethylene plays a crucial role in regulating fruit ripening, quality, and defense response. However, the mechanism (s) responsible for wound-induced ethylene regulation of fruit physiology at a network level is unclear. We used mass spectrometry (MS) to identify differences in the physiological response between fresh-cut fruits of wild-type (WT) tomato and an ethylene receptor mutant (SlETR-3) (also referred to as Nr) during storage. We found that Nr mutants exhibited better appearance and quality, as well as higher ethylene levels during the first 3 d of storage at 4 degrees C. Thirty-seven (0 h), eighty-two (12 h) and twelve (24 h) differentially abundant proteins were identified between the fresh-cut slices of the two genotypes during storage at the designated timepoints. In particular, antioxidant enzymes, such as ascorbate peroxidase, glutathione S-transferase, and peroxiredoxin were highly expressed in WT fruit, which was associated with higher H2O2 production, and high levels of transcription of cell -wall degrading enzymes. Leucine aminopeptidase, a marker enzyme for response to wounding exhibited higher levels in the Nr mutant, which is consistent with its higher production of ethylene. Collectively, our results provide a deeper insight into the ethylene-induced physiological regulatory network that is activated in fresh-cut tomatoes.

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