Molecular mechanisms of naringin's high lipid-lowering activity: pancreatic lipase inhibition and fat accumulation reduction

文献类型： 外文期刊

第一作者： Yu, Kai

作者： Yu, Kai;Jiang, Wenjing;Cheng, Ningning;Zhang, Ye;Wang, Hao;Li, Baorui;Zong, Aizhen;Xie, Jiao

作者机构：

关键词： naringin; pancreatic lipase (PL); inhibition mechanism; molecular simulation; anti-obesity

期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE （ 影响因子：3.5； 五年影响因子：4.2 ）

ISSN： 0022-5142

年卷期： 2025 年 105 卷 11 期

页码：

收录情况： SCI

摘要： BACKGROUNDNaringin, a natural flavonoid compound, has demonstrated potential anti-obesity effects by modulating lipid metabolism. Pancreatic lipase plays a critical role in lipid digestion and absorption, making it a key target for obesity management. This study investigated the inhibitory mechanism of naringin on pancreatic lipase through in vitro, in vivo, and in silico experiments.RESULTSIn vitro experiments revealed that naringin was a competitive inhibitor of pancreatic lipase, with a half-maximal inhibitory concentration (IC50) of 6.93 +/- 0.12 mmol/L. Multi-fluorescence spectroscopy confirmed that naringin induced secondary structure changes and static fluorescence quenching of pancreatic lipase, with a binding constant of 2.16 x 104 L/mol. Isothermal titration calorimetry indicated a spontaneous exothermic reaction (-26.08 kJ/mol), mainly driven by hydrogen bonding and electrostatic force. In vivo studies demonstrated that naringin decreased serum lipase activity by 24.54%, mitigated hepatic lipid accumulation, and down-regulated inflammatory cytokines in high-fat diet-induced obese rats. Molecular simulation further revealed that hydrogen bonding was the primary interaction force between naringin and pancreatic lipase.CONCLUSIONSNaringin effectively inhibits pancreatic lipase through competitive binding and structural modifications, reducing lipid digestion and absorption. The inhibitory effect of naringin on pancreatic lipase might provide new insights into the role of naringin in lipid metabolism regulation and potential application in obesity management. (c) 2025 Society of Chemical Industry.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序