Molecular and Cytogenetic Identification of Wheat-Thinopyrum intermedium Double Substitution Line-Derived Progenies for Stripe Rust Resistance

文献类型： 外文期刊

第一作者： Li, Guangrong

作者： Li, Guangrong;Chen, Qiheng;Jiang, Wenxi;Zhang, Ahui;Yang, Zujun;Yang, Ennian

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关键词： chromosome translocation; ND-FISH; Oligo-FISH painting; rust resistance; Thinopyrum intermedium; wheat

期刊名称：PLANTS-BASEL （ 影响因子：4.5； 五年影响因子：4.8 ）

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年卷期： 2023 年 12 卷 1 期

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收录情况： SCI

摘要： Thinopyrum intermedium (2n = 6x = 42, JJJ(S)J(S)StSt) has been hybridized extensively with common wheat and proven to be a valuable germplasm source for improving disease resistance and yield potential of wheat. A novel disease-resistant wheat-Th. intermedium double substitution line X479, carrying 1St(1B) and 4St-4J(S) (4B), was identified using multi-color non-denaturing fluorescence in situ hybridization (ND-FISH). With the aim of transferring Thinopyrum-specific chromatin to wheat, a total of 573 plants from F-2 and F-3 progenies of X479 crossed with wheat cultivar MY11 were developed and characterized using sequential ND-FISH with multiple probes. Fifteen types of wheat-Thinopyrum translocation chromosomes were preferentially transmitted in the progenies, and the homozygous wheat-1St, and wheat-4J(S)L translocation lines were identified using ND-FISH, Oligo-FISH painting and CENH3 immunostaining. The wheat-4J(S)L translocation lines exhibited high levels of resistance to stripe rust prevalent races in field screening. The gene for stripe rust resistance was found to be physically located on FL0-0.60 of the 4J(S)L, using deletion lines and specific DNA markers. The new wheat-Th. intermedium translocation lines can be exploited as useful germplasms for wheat improvement.

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