Differential DNA Methylation and Gene Expression Between ALV-J-Positive and ALV-J-Negative Chickens

文献类型： 外文期刊

第一作者： Yan, Yiming

作者： Yan, Yiming;Gao, Shuang;Zhang, Xinheng;Chen, Weiguo;Lin, Wencheng;Xie, Qingmei;Yan, Yiming;Gao, Shuang;Zhang, Xinheng;Chen, Weiguo;Lin, Wencheng;Xie, Qingmei;Yan, Yiming;Gao, Shuang;Zhang, Xinheng;Chen, Weiguo;Lin, Wencheng;Xie, Qingmei;Zhang, Huihua;Zhang, Huanmin

作者机构：

关键词： MeDIP-Seq; RNA-Seq; integrated analysis; TGFB2; ALV-J-positive chickens

期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.412； 五年影响因子：3.588 ）

ISSN：

年卷期： 2021 年 8 卷

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收录情况： SCI

摘要： Background: Avian leukosis virus subgroup J (ALV-J) is an oncogenic virus that causes serious economic losses in the poultry industry; unfortunately, there is no effective vaccine against ALV-J. DNA methylation plays a crucial role in several biological processes, and an increasing number of diseases have been proven to be related to alterations in DNA methylation. In this study, we screened ALV-J-positive and -negative chickens. Subsequently, we generated and provided the genome-wide gene expression and DNA methylation profiles by MeDIP-seq and RNA-seq of ALV-J-positive and -negative chicken samples; 8,304 differentially methylated regions (DMRs) were identified by MeDIP-seq analysis (p <= 0.005) and 515 differentially expressed genes were identified by RNA-seq analysis (p <= 0.05). As a result of an integration analysis, we screened six candidate genes to identify ALV-J-negative chickens that possessed differential methylation in the promoter region. Furthermore, TGFB2 played an important role in tumorigenesis and cancer progression, which suggested TGFB2 may be an indicator for identifying ALV-J infections.

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