Synergistic Control of Seed Germination and Seedling Establishment by UBP1A and UBP1C via ABA Signaling in Arabidopsis

文献类型： 外文期刊

第一作者： Shi, Huiying

作者： Shi, Huiying;Jing, Meng;Liu, Yanan;Zheng, Lei;Han, Yuzhen;Yan, Zongyun;Shi, Huiying

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关键词： Arabidopsis; UBP1A; UBP1C; ABA; Seed germination; RNA-binding proteins

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.4； 五年影响因子：4.9 ）

ISSN： 0721-7595

年卷期： 2025 年

页码：

收录情况： SCI

摘要： RNA-binding proteins (RBPs) play essential roles in plant development, growth, and environmental responses. UBP1A and UBP1C are oligouridylate-binding proteins containing three RNA recognition motifs (RRMs), but their functions during early plant development remain largely unknown. In this study, we used CRISPR/Cas9 to generate UBP1A and UBP1C knockout mutants to investigate their roles. Phenotypic analysis revealed that the ubp1a ubp1c (ubp1ac) double mutant exhibited delayed seed germination and reduced cotyledon greening, while the ubp1a and ubp1c single mutants showed no obvious phenotypic changes. These findings suggest that UBP1A and UBP1C function redundantly and positively in regulating early seedling development. Furthermore, ubp1ac exhibited hypersensitivity to ABA, along with upregulation of ABA biosynthesis and signaling genes. Expression analysis showed that UBP1A and UBP1C are upregulated in imbibed seeds and induced by ABA during early germination. Subcellular localization analysis indicated that UBP1A and UBP1C are distributed in both the cytoplasm and nucleus, and relocalize into cytoplasmic granules in response to ABA, suggesting their involvement in stress granule (SG) dynamics. Together, these results indicate that UBP1A and UBP1C cooperatively and positively regulate seed germination and cotyledon greening and may act as negative feedback regulators of ABA signaling to promote early developmental progression in Arabidopsis.

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