TPLATE complex-dependent endocytosis attenuates CLAVATA1 signaling for shoot apical meristem maintenance

文献类型： 外文期刊

第一作者： Wang, Jie

作者： Wang, Jie;Jiang, Qihang;Pleskot, Roman;Grones, Peter;Xu, Xiangyu;Vandorpe, Michael;Mylle, Evelien;De Smet, Ive;Vernoux, Teva;Nowack, Moritz K.;Van Damme, Daniel;Wang, Jie;Jiang, Qihang;Pleskot, Roman;Grones, Peter;Xu, Xiangyu;Vandorpe, Michael;Mylle, Evelien;De Smet, Ive;Nowack, Moritz K.;Van Damme, Daniel;Wang, Jie;Pleskot, Roman;Bahafid, Elmehdi;Denay, Gregoire;Simon, Ruediger;Bahafid, Elmehdi;Denay, Gregoire;Simon, Ruediger;Galvan-Ampudia, Carlos

作者机构：

关键词： clathrin-mediated endocytosis; CLAVATA3-CLAVATA1 signaling; shoot apical meristem maintenance; TPLATE complex

期刊名称：EMBO REPORTS （ 影响因子：7.7； 五年影响因子：8.6 ）

ISSN： 1469-221X

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Endocytosis regulates the turnover of cell surface localized receptors, which are crucial for plants to rapidly respond to stimuli. The evolutionary ancient TPLATE complex (TPC) plays an essential role in endocytosis in Arabidopsis plants. Knockout or knockdown of single TPC subunits causes male sterility and seedling lethality phenotypes, complicating analysis of the roles of TPC during plant development. Partially functional alleles of TPC subunits however only cause mild developmental deviations. Here, we took advantage of the partially functional TPLATE allele, WDXM2, to investigate a role for TPC-dependent endocytosis in receptor-mediated signaling. We discovered that reduced TPC-dependent endocytosis confers a hypersensitivity to very low doses of CLAVATA3 peptide signaling. This hypersensitivity correlated with the abundance of the CLAVATA3 receptor protein kinase CLAVATA1 at the plasma membrane. Genetic and biochemical analysis as well as live-cell imaging revealed that TPC-dependent regulation of CLAVATA3-dependent internalization of CLAVATA1 from the plasma membrane is required for shoot stem cell homeostasis. Our findings provide evidence that TPC-mediated endocytosis and degradation of CLAVATA1 is a mechanism to dampen CLAVATA3-mediated signaling during plant development.

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