Silencing gram-negative bacteria binding protein 1 decreases the immunity of Tribolium castaneum against bacteria

文献类型： 外文期刊

第一作者： Bi, Jingxiu

作者： Bi, Jingxiu;Liu, Pingxiang;Gao, Rui;Jiang, Yuying;Zhang, Chao;Zhao, Tong;Gao, Lei;Wang, Yutao;Wang, Yutao

作者机构：

关键词： Tribolium castaneum; Gram-negative bacteria binding protein; Innate immunity; Molecular docking; Pest management

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2024 年 264 卷

页码：

收录情况： SCI

摘要： Gram-negative bacteria binding proteins (GNBPs) have the ability to recognize molecular patterns associated with microbial pathogens (PAMPs), leading to the activation of immune responses downstream. In the genome of Tribolium castaneum , three GNBP genes have been identified; however, their immunological roles remain unexplored. In our study, a GNBP1 , designated as TcGNBP1 , were identified from the cDNA library of T. castaneum . The coding sequence of TcGNBP1 consisted of 1137 bps and resulted in the synthesis of a protein comprising 378 amino acids. This protein encompasses a signal peptide, a low -complexity region, and a glycoside hydrolase 16 domain. TcGNBP1 was strongly expressed in early adult stages, and mainly distributed in hemolymph and gut. Upon being challenged with Escherichia coli or Staphylococcus aureus , the transcript levels of TcGNBP1 were significantly changed at different time points. Through molecular docking and ELISA analysis, it was observed that TcGNBP1 has the ability to interact with lipopolysaccharides, peptidoglycan, and 13- 1, 3-glucan. Based on these findings, it was further discovered that recombinant TcGNBP1 can directly bind to five different bacteria in a Ca 2 + -dependent manner. After knockdown of TcGNBP1 with RNA interference, expression of antimicrobial peptide genes and prophenoloxidase (proPO) activity were suppressed, the susceptibility of T. castaneum to E. coli or S. aureus infection was enhanced, leading to low survival rate. These results suggest a regulatory mechanism of TcGNBP1 in innate immunity of T. castaneum and provide a potential molecular target for dsRNA-based insect pest management.

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