The role of m6A RNA methylation in a love-hate relationship between porcine rotavirus and host cells

文献类型： 外文期刊

第一作者： Liang, Yaxu

作者： Liang, Yaxu;Zhuo, Ruhao;Peng, Ning;Gan, Zhending;Qi, Jun;Wang, Zhibo;Zhong, Xiang;Zhong, Xiang;Zhu, Xuejiao;Chen, Shuyu;Li, Bin;Zhuo, Ruhao;Huang, Shimeng

作者机构：

关键词： N6-methyladenosine; Porcine rotavirus; Immune; IRF2; IFI44L

期刊名称：CELL AND BIOSCIENCE （ 影响因子：6.2； 五年影响因子：7.2 ）

ISSN：

年卷期： 2025 年 15 卷 1 期

页码：

收录情况： SCI

摘要： N6-methyladenosine (m(6)A), the most abundant mRNA modification, regulates various mRNA metabolism to affect numerous physiological processes, including immune response. Interestingly, many RNA viruses contain internal m(6)A modifications that contribute to viral replication and innate immune escape process, but its mechanisms remain unclear. Porcine rotavirus (PoRV) is a common cause of diarrhea and gastroenteritis in piglets. Here, we first revealed the m(6)A methylation profile on the PoRV genome. PoRV infection significantly reduced methyltransferase METTL3 expression and induced nuclear-cytoplasmic translocation of METTL3. The structural protein VP6 of PoRV can co-localize with METTL3 in the cytoplasm and bind to METTL3 protein, suggesting that PoRV hijacked the host METTL3 to achieve m(6)A methylation. On the contrary, knockdown of Mettl3 or Ythdf2 in IPEC cells inhibited the replication of PoRV. Mechanistically, silencing of Mettl3 or Ythdf2 enhanced the expression of IRF2 and IFI44L via an increase of mRNA stability of Irf2 and Ifi44l. Furthermore, knockdown of Irf2 and Ifi44l promoted viral replication in IPEC cells. In conclusion, PoRV took full advantage of METTL3 to promote replication, in turn, host reduced own m(6)A methylation to enhance IRF2 and IFI44L to restrain virus infection, suggesting a love-hate relationship between virus and host, and providing novel targets for developing antiviral drugs in the pig industry.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序