Halomonas sp. NEC-1, an ectoine-rich and wide pH tolerant halophilic bacterium isolated from a salt lake of Xizang, China

文献类型： 外文期刊

第一作者： Ma, Yingchao

作者： Ma, Yingchao;Sun, Zhongshi;Li, Yukang;Zou, Hanying;Zhao, Yuanliang;Lin, Feiyang;Han, Xuekai;Sui, Liying;Han, Xuekai;Zhang, Chi

作者机构：

关键词： halophilic microorganism; ectoine; salt lake; Xizang

期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.3； 五年影响因子：1.5 ）

ISSN： 2096-5508

年卷期： 2025 年 43 卷 5 期

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收录情况： SCI

摘要： A halophilic bacterium, named Halomonas sp. NEC-1 was isolated from the Nyer Co Salt Lake on the Xizang Plateau, SW China. The strain exhibited a broad pH tolerance range of 5-11, distinguishing it from Halomonas alkaliphila despite a 99% genetic similarity. Optimal growth conditions, determined through orthogonal experiments, were found to be 37 degrees C, 100-g/L salinity, and an initial pH of 6, resulting in a maximum OD600 of 7.98 +/- 0.06. Halomonas sp. NEC-1 produced 545.43 +/- 25.10 mg/L of ectoine under optimal conditions of 75-g/L salinity, 40-g/L sodium glutamate, and an initial pH of 6. This production increased to 1 388.81 +/- 3.69 mg/L after five rounds of hypo-osmotic shocks. During the shocks, ectoine productivity remained stable at approximately 16.29 +/- 0.04 to 17.28 +/- 0.48 mg/(Lh), representing a 43.40%-52.11% increase compared to the rate without any shock (11.36 +/- 1.05 mg/(Lh)). Additionally, the expression of the ectABC gene cluster, related to ectoine synthesis, significantly increased following the shocks, enhancing ectoine production. The ectoine extract demonstrated notable protective effects on Escherichia coli and plasmid DNA. After 10 min of exposure at 60 degrees C, the colony count of E. coli treated with ectoine extract increased by 342% compared to treatment with distilled water. Furthermore, the ectoine extract protected plasmid DNA from 2,2 '-Azobis (2-methylpropionamidine) dihydrochloride-induced damage. This study highlights Halomonas sp. NEC-1 is a promising strain for ectoine production and underscores the potential of microbial resources in salt lakes from Xizang region.

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