A single dose of an ALVAC vector-based RABV virus-like particle candidate vaccine induces a potent immune response in mice, cats and dogs

文献类型： 外文期刊

第一作者： Meng, Xianyong

作者： Meng, Xianyong;Cong, Haiyang;Li, Jiaqi;Wang, Jianzhong;Feng, Na;Xia, Xianzhu;Meng, Xianyong;Yan, Feihu;Wang, Weiqi;Wang, Shen;Cong, Haiyang;Li, Jiaqi;Zhao, Yongkun;Wang, Tiecheng;Li, Nan;Gao, Yuwei;Feng, Na;Xia, Xianzhu;Yan, Feihu;Wang, Shen;Zhao, Yongkun;Wang, Tiecheng;Li, Nan;Feng, Na;Xia, Xianzhu;Wang, Weiqi

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关键词： Rabies virus; canarypox-virus vector; CRISPR/Cas9; virus-like particles; cats; dogs

期刊名称：EMERGING MICROBES & INFECTIONS （ 影响因子：7.5； 五年影响因子：7.2 ）

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年卷期： 2024 年 13 卷 1 期

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收录情况： SCI

摘要： Rabies, caused by the Rabies virus (RABV), is a highly fatal zoonotic disease. Existing rabies vaccines have demonstrated good immune efficacy, but the complexity of immunization procedures and high cost has impeded the elimination of RABV, particularly in the post-COVID-19 era. There is a pressing need for safer and more effective rabies vaccines that streamline vaccination protocols and reduce expense. To meet this need, we have developed a potential rabies vaccine candidate called ALVAC-RABV-VLP, utilizing CRISPR/Cas9 gene editing technology. This vaccine employs a canarypox virus vector (ALVAC) to generate RABV virus-like particles (VLPs). In mice, a single dose of ALVAC-RABV-VLP effectively activated dendritic cells (DCs), follicular helper T cells (Tfh), and the germinal centre (GC)/plasma cell axis, resulting in durable and effective humoral immune responses. The survival rate of mice challenged with lethal RABV was 100%. Similarly, in dogs and cats, a single immunization with ALVAC-RABV-VLP elicited a stronger and longer-lasting antibody response. ALVAC-RABV-VLP induced superior cellular and humoral immunity in both mice and beagles compared to the commercial inactivated rabies vaccine. In conclusion, ALVAC-RABV-VLP induced robust protective immune responses in mice, dogs and cats, offering a novel, cost-effective, efficient, and promising approach for herd prevention of rabies.

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