Validation and Application of a Molecular Detection System for Fusarium Wilt of Banana in China

文献类型： 外文期刊

第一作者： Yang, Di

作者： Yang, Di;Du, Chanjuan;Zhang, Jin;Pan, Lianfu;Wei, Shaolong;Jiang, Shangbo;Fu, Gang;Li, Chunyu;San, Cho Cho;Huy, Nguyen Duc;Ye, Yunfeng

作者机构：

关键词： Fusarium oxysporum f. sp. cubense; molecular detection; primer evaluation

期刊名称：PLANT DISEASE （ 影响因子：4.5； 五年影响因子：5.0 ）

ISSN： 0191-2917

年卷期： 2023 年 107 卷 12 期

页码：

收录情况： SCI

摘要： Fusarium wilt of banana is a devastating disease caused by Fusarium oxysporum f. sp. cubense (Foc). It has restricted the development of the banana industry worldwide and is particularly serious in China because of the large planting areas and special planting patterns. However, there is no rapid and accurate approach to detect the Foc strains that specifically occur in China because of the rich genetic diversity observed in this pathosystem. In this study, we evaluated the performance of 10 previously published PCR primer pairs on 103 representative Foc strains in China and neighboring countries and screened out a set of primers (Foc-specific primer pair SIX9-Foc-F/R, Foc R1-specific primer pair SIX6b-210-F/R, Foc R4-specific primer pair Foc-1/2, and Foc TR4-specific primer pair W2987F/R) suitable for the detection of Foc strains in China and the surrounding Southeast Asian countries. Moreover, we developed a molecular detection system to accurately identify the different physiological races of Foc. The findings of this study provide technical support for preventing and controlling the spread of Fusarium wilt of banana in the field in China.

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