Pijx confers broad-spectrum seedling and panicle blast resistance by promoting the degradation of ATP β subunit and OsRbohC-mediated ROS burst in rice

文献类型： 外文期刊

第一作者： Xiao, Ning

作者： Xiao, Ning;Wu, Yunyu;Zhang, Xiaoxiang;Chen, Zichun;Cai, Yue;Yu, Ling;Wang, Zhiping;Shi, Wei;Pan, Cunhong;Li, Yuhong;Zhou, Changhai;Liu, Jianju;Huang, Niansheng;Liu, Guangqing;Ji, Hongjuan;Zhu, Shuhao;Fang, Shuai;Li, Aihong;Hao, Zeyun;Wang, Ruyi;Ning, Yuese;Yang, Zefeng;Lu, Yue

作者机构：

关键词： panicle blast; broad-spectrum resistance; Pijx; ATPb; ROS burst

期刊名称：MOLECULAR PLANT （ 影响因子：27.5； 五年影响因子：22.6 ）

ISSN： 1674-2052

年卷期： 2023 年 16 卷 11 期

页码：

收录情况： SCI

摘要： Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most important diseases of rice. Utilization of blast-resistance genes is the most economical, effective, and environmentally friendly way to control the disease. However, genetic resources with broad-spectrum resistance (BSR) that is effective throughout the rice growth period are rare. In this work, using a genome-wide association study, we identify a new blast-resistance gene, Pijx, which encodes a typical CC-NBS-LRR protein. Pijx is derived from a wild rice species and confers BSR to M. oryzae at both the seedling and panicle stages. The functions of the resistant haplotypes of Pijx are confirmed by gene knockout and overexpression experiments. Mechanistically, the LRR domain in Pijx interacts with and promotes the degradation of the ATP synthase beta subunit (ATPb) via the 26S proteasome pathway. ATPb acts as a negative regulator of Pijx-mediated panicle blast resistance, and interacts with OsRbohC to promote its degradation. Consistently, loss of ATPb function causes an increase in NAPDH content and ROS burst. Remarkably, when Pijx is introgressed into two japonica rice varieties, the introgression lines show BSR and increased yields that are approximately 51.59% and 79.31% higher compared with those of their parents in a natural blast disease nursery. In addition, we generate PPLPijx Pigm and PPLPijx Piz-t pyramided lines and these lines also have higher BSR to panicle blast compared with Pigm- or Piz-t-containing rice plants. Collectively, this study demonstrates that Pijx not only confers BSR to M. oryzae but also maintains high and stable rice yield, providing new genetic resources and molecular targets for breeding rice varieties with broad-spectrum blast resistance.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序