High sensitive and selective electrochemical aptasensor for patulin detection based on a novel assembling strategy using hierarchically porous carbon

文献类型： 外文期刊

第一作者： Jiang, Cuiwen

作者： Jiang, Cuiwen;Wang, Yanli;Liang, Jing;Xie, Liping;Liang, Zhongdan;Li, Huiling;Li, Tao;Ning, Dejiao;Tang, Li;Huang, Xiaochuan;Luo, Lihong;Ya, Yu;Yan, Feiyan;Huang, Kejing

作者机构：

关键词： Patulin; Hierarchically porous carbon; Aptasensor; Electroanalysis; Voltammetry

期刊名称：JOURNAL OF FOOD COMPOSITION AND ANALYSIS （ 影响因子：4.3； 五年影响因子：4.6 ）

ISSN： 0889-1575

年卷期： 2024 年 131 卷

页码：

收录情况： SCI

摘要： Patulin (PAT) in food is a menace to human health. Hence, a sensitive and precise approach to PAT detection is significant. In this research, an innovative electrochemical assay was developed for sensitively determining PAT by assembling aptamers on a carboxylation hierarchically porous carbon (HPC-COOH) modified glassy carbon electrode. Diverse analytical methods were used to describe electrode morphology, structure, chemical composition, and electrochemistry properties. The HPC-COOH, with a large surface area, played a crucial role as a unique substrate to effectively immobilize aptamers and enhance target PATs ' recognition and capture ability. Simultaneously, the excellent electrical conductivity of HPC-COOH was significant for the signal amplification of sensors. Under optimized conditions, the aptasensor enabled efficient PAT detection in a broad concentration range (0.5 - 5.0 x10 6 ng/L) with a low detection limit (0.25 ng/L). The designed aptasensor showed high sensitivity, selectivity, reproducibility, and stability under optimized conditions. The sensors ' feasibility for practical applications was further demonstrated by analyzing apple juice and haw juice samples.

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