A highly branched glucomannan from the fruiting body of Schizophyllum commune: Structural characteristics and antitumor properties analysis

文献类型： 外文期刊

第一作者： Gao, Hong

作者： Gao, Hong;Shi, Defang;Yin, Chaomin;Fan, Xiuzhi;Yao, Fen;Qiu, Jianhui;Yu, Wei;Gao, Hong;Shi, Defang;Yin, Chaomin;Fan, Xiuzhi;Yao, Fen;Qiu, Jianhui;Yu, Wei;Cheng, Xianbo;Qiao, Xin;Liu, Chunyou;Hu, Guoyuan

作者机构：

关键词： Schizophyllum commune; Glucomannan; Structural characterization; Antitumor activity; Apoptosis induction

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2024 年 282 卷

页码：

收录情况： SCI

摘要： In this study, a highly branched glucomannan (SCP-1) from Schizophyllum commune fruiting body with good solubility was isolated, and its structural characteristics and antitumor properties were analyzed. The monosaccharides of SCP-1 were fucose, glucosamine hydrochloride, galactose, glucose and mannose with a relative molar ratio of 14:6:210:593:177, and the molecular weight (Mw) of SCP-1 was 15.1 kDa. SCP-1 showed a rough and dense surface, and it was aggregated to particles in distilled water, though it might have triple-helix conformation. The main backbone chain of SCP-1 was -*[3)-(3-D-Glcp-(1]3-*3)-(3-D-Glcp-(1-*2)-alpha-D-Manp(1-*2)-alpha-D-Manp-(1-*3)-alpha-D-Glcp-(1-* and three sides chains including alpha-D-Glcp-(1-*[6)-(3-D-Glcp-(1]2-*, alpha-DGlcp-(1-*3)-alpha-D-Manp-(1-* and alpha-D-Glcp-(1-*[6)-alpha-D-Galp-(1]3-* were linked with 1,6-glycosidic bond, which was significantly different with the schizophyllan isolated from the mycelia of S. commune. SCP-1 could significantly inhibit the growth of A549 cells, the inhibition rate reached 41.62 % and the percentage of cells in S phase increased from 27.17 % to 56.40 % (400 mu g/mL, 48 h). Moreover, SCP-1 could induce cell apoptosis and the total apoptosis rate reached 28.13 %. SCP-1 exerted apoptosis inducing effect probably by reducing the expression ratio of Bcl-2/Bax and the p-PI3K, p-Akt and p-mTOR expression level. The results showed that SCP-1 might have the potential to act as an antitumor agent for lung cancer therapy.

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