DNA methylation provides new insights into the epigenetic regulation of Vibrio parahaemolyticus infection in Portunus trituberculatus

文献类型： 外文期刊

第一作者： Sun, Dongfang

作者： Sun, Dongfang;Zhou, Xianfa;Jia, Shaoting;Liu, Ping;Lv, Jianjian;Liu, Ping;Lv, Jianjian

作者机构：

关键词： Crustacean; Vibrio parahaemolyticus; DNA methylation; WGBS

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：4.0 ）

ISSN： 2352-5134

年卷期： 2025 年 41 卷

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收录情况： SCI

摘要： Vibrio parahaemolyticus is the primary pathogen responsible for acute hepatopancreatic necrotic disease in crustaceans. Research on pathogenic mechanism of V. parahaemolyticus in crustaceans has predominantly concentrated on genetics, with limited exploration of epigenetic regulatory mechanisms. Here, to elucidate the epigenetic regulatory mechanisms of crustacean resistance to V. parahaemolyticus infection, we conducted an artificial infection study on P. trituberculatus. The results indicated a significant increase in the expression of DNA methyltransferase Dnmt1 following 72 h of V. parahaemolyticus infection. Subsequently, we depict for the first time DNA methylation profiles of the whole genome of P. trituberculatus at single-base resolution by using WGBS on hepatopancreatic tissues. The overall DNA methylation level was low at 2.2 % in P. trituberculatus. Methylation sites exhibited a mosaic distribution across the genome, predominantly of the CG type, in line with other invertebrates. The DNA methylation of P. trituberculatus changed significantly after pathogen infection, showing localized hypermethylation or hypomethylation. A total of 1832 DMRs, with 1072 being hypermethylated and 766 hypomethylated were identified. The DMRs were annotated across 1005 genes. Differently methylated genes were significantly enriched in immune-related signaling pathways, including enrichment in the NF-kappa beta signaling pathway and platelet activation. Finally, we verified the inhibitory effect of DNA methylation on the expression of the immune-related gene Dad1 using a dual luciferase reporter assay. These findings will enhance our understanding of the regulatory mechanisms in crustacean innate immunity and offer epigenetic markers for breeding disease-resistant P. trituberculatus varieties, presenting significant theoretical and practical value.

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