To explore the regulatory effect of Buyang Huanwu Decoction on cerebral infarction based on quantitative proteomics

文献类型： 外文期刊

第一作者： Li, Ying

作者： Li, Ying;Miao, Lan;Sun, Mingqian;Lin, Li;Ren, Junguo;Liu, Jianxun;Guo, Rongjuan;He, Lijuan;Pan, Yinghong

作者机构：

关键词： Cerebral infarction; Buyang Huanwu Decoction; Qi deficiency and blood stasis syndrome; Proteomics; LC-MS; MS

期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：3.3； 五年影响因子：3.5 ）

ISSN： 1874-3919

年卷期： 2023 年 277 卷

页码：

收录情况： SCI

摘要： Buyang Huanwu Decoction (BYHW) contains chemical components such as ligustrazine, oxypaeoniflora, chlorogenic acid, and others. To explore the neuroprotective effect and potential target protein of BYHW in cerebral infarction (CI). A double-blind, randomized controlled trial was established and patients with CI were divided into the BYHW group (n = 35) and the control group (n = 30). To evaluate the efficacy by TCM syndrome score and clinical indicators, and to explore the changes of serum proteins by proteomics technology, so as to explore the mechanism of BYHW and potential target proteins. The study found that compared with the control group, the TCM syndrome score, including Deficiency of Vital Energy (DVE), Blood Stasis (BS), and NIHSS in the BYHW group decreased significantly (p < 0.05), and the Barthel Index (BI) score was significantly higher. A total of 99 differential regulatory proteins were identified by proteomics, which act on lipids and atherosclerosis, complement and coagulation cascade, and TNF-alpha signaling pathway. In addition, Elisa verified the results of proteomics and found that BYHW can reduce the neurological impairments focus on IL-1 beta, IL-6, TNF-alpha, MCP-1, MMP-9, and PAI-1. Significance: In this study, quantitative proteomics was used in combination with liquid chromatography-mass spectrometry (LC-MS/MS) to study the therapeutic effect of BYHW on cerebral infarction (CI) and potential changes in serum proteomics. In addition, the public proteomics database was used for bioinformatics analysis, and Elisa experiment verified the results of proteomics, further clarifying the potential protection mechanism of BYHW on CI.

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