Phytopathogenic bacteria utilize host glucose as a signal to stimulate virulence through LuxR homologues

文献类型： 外文期刊

第一作者： Zhang, Siyuan

作者： Zhang, Siyuan;Kan, Jinhong;Liu, Xin;Wu, Yao;Zhang, Mingyang;Ou, Jinqing;Wang, Juan;An, Lin;Li, Defeng;Wang, Li;Fang, Rongxiang;Jia, Yantao;Zhang, Siyuan;Kan, Jinhong;Ou, Jinqing;Liu, Xin;Wang, Xiu-Jie;Jia, Yantao;Kan, Jinhong

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关键词： glucose; interkingdom signalling; LuxR ligand; quorum sensing

期刊名称：MOLECULAR PLANT PATHOLOGY （ 影响因子：4.9； 五年影响因子：5.9 ）

ISSN： 1464-6722

年卷期： 2023 年 24 卷 4 期

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收录情况： SCI

摘要： Chemical signal-mediated biological communication is common within bacteria and between bacteria and their hosts. Many plant-associated bacteria respond to unknown plant compounds to regulate bacterial gene expression. However, the nature of the plant compounds that mediate such interkingdom communication and the underlying mechanisms remain poorly characterized. Xanthomonas campestris pv. campestris (Xcc) causes black rot disease on brassica vegetables. Xcc contains an orphan LuxR regulator (XccR) which senses a plant signal that was validated to be glucose by HPLC-MS. The glucose concentration increases in apoplast fluid after Xcc infection, which is caused by the enhanced activity of plant sugar transporters translocating sugar and cell-wall invertases releasing glucose from sucrose. XccR recruits glucose, but not fructose, sucrose, glucose 6-phosphate, and UDP-glucose, to activate pip expression. Deletion of the bacterial glucose transporter gene sglT impaired pathogen virulence and pip expression. Structural prediction showed that the N-terminal domain of XccR forms an alternative pocket neighbouring the AHL-binding pocket for glucose docking. Substitution of three residues affecting structural stability abolished the ability of XccR to bind to the luxXc box in the pip promoter. Several other XccR homologues from plant-associated bacteria can also form stable complexes with glucose, indicating that glucose may function as a common signal molecule for pathogen-plant interactions. The conservation of a glucose/XccR/pip-like system in plant-associated bacteria suggests that some phytopathogens have evolved the ability to utilize host compounds as virulence signals, indicating that LuxRs mediate an interkingdom signalling circuit.

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