A soybean NAC homolog contributes to resistance to Phytophthora sojae mediated by dirigent proteins

文献类型： 外文期刊

第一作者： Yu, Guolong

作者： Yu, Guolong;Zou, Jianan;Wang, Jinhui;Zhu, Rongsheng;Qi, Zhaoming;Hu, Zhenbang;Yang, Mingliang;Zhao, Ying;Wu, Xiaoxia;Liu, Chunyan;Li, Candong;Chen, Qingshan;Xin, Dawei;Jiang, Hongwei;Zhu, Zhendong;Fu, Yongfu;Jiang, Hongwei;Yu, Guolong;Li, Candong;Yang, Xue

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关键词： Phytophthora sojae; Soybean; GmNAC1; Dirigent; Lignin

期刊名称：CROP JOURNAL （ 影响因子：4.647； 五年影响因子：5.781 ）

ISSN： 2095-5421

年卷期： 2022 年 10 卷 2 期

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收录情况： SCI

摘要： Phytophthora sojae infection severely impairs soybean production. We previously identified a dirigent protein, GmDRR1 (Glycine max Disease Resistant Response 1), that increases soybean resistance to P. sojae. However, the molecular basis of GmDRR1 function remained largely uncharacterized. In the present study, analysis of GmDRR1-RNAi, GmDRR1-overexpressing, and CRISPR/Cas9-derived Gmdrr1 mutant lines revealed that GmDRR1 expression significantly restricted P. sojae growth. Combining co-immunoprecipitation with liquid chromatography-tandem mass spectrometry revealed a GmDRR1-interacting protein, GmDRR2, which is homologous to GmDRR1. An E-coniferyl alcohol coupling assay indicated that GmDRR1 promotes the synthesis of (+)-pinoresinol, which helps to protect plants from P. sojae. The GmNAC1 (Glyma.05G025500) transcription factor bound to the GmDRR1 promoter both in vitro and in vivo to upregulate GmDRR1 expression. Soybean resistance to P. sojae was increased by overexpression of GmNAC1. Our findings suggest a novel signaling pathway involving a NAC transcription factor that mediates soybean resistance to P. sojae. Specifically, GmNAC1 directly induces GmDRR1 expression to increase resistance of soybean plants to P. sojae. (C) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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