Heat shock protein 71 restricts mutation of porcine reproductive and respiratory syndrome virus nsp2 in vitro

文献类型： 外文期刊

第一作者： Xie, Fengyu

作者： Xie, Fengyu;Li, Manxiang;Xie, Fengyu;Kang, Lei;Chen, Mengli;Zhang, Tong;Li, Zongjie;Shao, Donghua;Li, Beibei;Wei, Jianchao;Qiu, Yafeng;Ma, Zhiyong;Liu, Ke;Kang, Lei;Chen, Mengli

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关键词： Porcine reproductive and respiratory syndrome; virus nsp2; Heat shock protein 71; Mutation

期刊名称：COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES （ 影响因子：2.0； 五年影响因子：2.3 ）

ISSN： 0147-9571

年卷期： 2024 年 109 卷

页码：

收录情况： SCI

摘要： porcine reproductive and respiratory syndrome (PRRS), caused by porcine reproductive and respiratory syndrome virus (PRRSV) infection, is an important swine infectious disease that causes substantial losses worldwide each year. PRRSV is a positive-sense single-stranded RNA virus that is highly susceptible to mutation and recombination, making vaccine and drug research for the disease extremely difficult. In this study, the binding of PRRSV nsp2 to HSP71 protein was detected by using the IP/MS technique. And the inhibitory effect of HSP71 on nsp2 antagonistic activity was validated by measuring NF-kB luciferase reporter. According to stress from inhibitory effects, the amino acid variation profile of PRRSV nsp2 under HSP71 stress was further analyzed using second-generation sequencing. Surprisingly, the results indicated that HSP71 pressure limits the random mutations of PRRSV nsp2 and maintains the dominant PRRSV strain within the population. Mutant strain showed weaker antagonistic activity and replication capability in cell. These results imply the binding of HSP71 with PRRSV nsp2 may lead to maintain the stability of highly virulent strains of PRRSV.

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