The ZcVg3 Gene Regulates the Reproduction and Lifespan of Female Zeugodacus cucurbitae (Coquillett) Mediated by Short-Term High Temperatures

文献类型： 外文期刊

第一作者： Yang, Shuyan

作者： Yang, Shuyan;Peng, Sihua;Wang, Aqiang;Wu, Qianxing;Yang, Xiaofeng;Zhou, Shihao;Jia, Jingjing

作者机构：

关键词： Zeugodacus cucurbitae (Coquillett); RNA interference; Vitellogenin-3; reproduction; lifespan; synergistic relationship

期刊名称：INSECTS （ 影响因子：2.7； 五年影响因子：2.9 ）

ISSN：

年卷期： 2024 年 15 卷 7 期

页码：

收录情况： SCI

摘要： Exposure to short-term high temperatures of 45 degrees C can enhance the development and reproduction of Zeugodacus cucurbitae (Coquillett). In this study, the function of the ZcVg3 gene in reproduction was studied by interfering with its expression via the injection of siRNA. The silencing of the ZcVg3 gene could affect the expression of other related genes. It also resulted in a significant reduction in egg production, spawning days, ovarian development and lifespan. The results suggest that the ZcVg3 gene plays a crucial role in pest reproduction under short-term high temperatures. Zeugodacus cucurbitae (Coquillett) is a significant pest affecting fruit and vegetables in tropical and subtropical regions, and its development and reproduction are enhanced after exposure to short-term high-temperature stress at 45 degrees C. Vitellogenin (Vg) is an essential precursor of yolk protein formation in eggs and plays a vital role in the ovarian development of insects. Interfering with the Z. cucurbitae vitellogenin receptor (ZcVgR) gene in short-term high-temperature conditions decreases the fecundity of female adults, while the transcription level of the ZcVg3 gene increases. To elucidate the reproductive function of the ZcVg3 gene and the synergistic relationship among the ZcVgs genes under short-term high temperatures, this study injected siRNA to interfere with the ZcVg3 gene after subjecting Z. cucurbitae to a 1 h treatment at 45 degrees C and 25 degrees C. The expression of the ZcVg3 gene was suppressed, leading to the upregulation of the ZcVg1 and ZcVg2 genes, and the expression of the ZcVgR gene was initially decreased and then increased. Silencing the ZcVg3 gene after a 1 h treatment at 45 degrees C resulted in a reduction of approximately 84.7% and 75.9% in the fecundity and spawning days of female adults compared to the control. The development rate of their ovaries and the ovarian diameter significantly decreased, and their lifespan was reduced by 71%. The ZcVg3 gene plays a crucial role in the reproduction of Z. cucurbitae in short-term high-temperature conditions. The results of this study provide potential targets for the development of RNAi-based techniques for the control of Z. cucurbitae.

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