Identification of HQT gene family and their potential function in CGA synthesis and abiotic stresses tolerance in vegetable sweet potato

文献类型： 外文期刊

第一作者： Medison, Milca Banda

作者： Medison, Milca Banda;Pan, Rui;Peng, Ying;Medison, Rudoviko Galileya;Zhang, Wenying;Shalmani, Abdullah;Yang, XinSun

作者机构： Yangtze Univ, Res Ctr Crop Stresses Resistance Technol, Hubei Collaborat Innovat Ctr Grain Ind, Jingzhou 434025, Peoples R China;Northwest A&F Univ, Coll Life Sci, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Peoples R China;Hubei Acad Agr Sci, Inst Food Crops, Hubei Engn & Technol Res Ctr Sweet Potato, Hubei Key Lab Food Crop Germplasm & Genet Improvem, Wuhan 430064, Peoples R China

关键词： Ipomoea batatas; Abiotic stress; Chlorogenic acid; Hydroxycinnamate-CoA quinate hydroxycinnamoyl transferase

期刊名称：PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS （ 2022影响因子：3.5； 五年影响因子：3.9 ）

ISSN： 0971-5894

年卷期： 2023 年 29 卷 3 期

页码：

收录情况： SCI

摘要： Hydroxycinnamate-CoA quinate hydroxycinnamoyl transferase (HQT) enzyme affect plant secondary metabolism and are crucial for growth and development. To date, limited research on the genome-wide analysis of HQT family genes and their regulatory roles in chlorogenic acid (CGA) accumulation in leafy vegetable sweet potato is available. Here, a total of 58 HQT family genes in the sweet potato genome (named IbHQT) were identified and analyzed. We studied the chromosomal distribution, phylogenetic relationship, motifs distribution, collinearity, and cis-acting element analysis of HQT family genes. This study used two sweet potato varieties, high CGA content Fushu 7-6-14-7 (HC), and low CGA content Fushu 7-6 (LC). Based on the phylogenetic analysis, clade A was unique among the identified four clades as it contained HQT genes from various species. The chromosomal location and collinearity analysis revealed that tandem gene duplication may promote the IbHQT gene expansion and expression. The expression patterns and profile analysis showed changes in gene expression levels at different developmental stages and under cold, drought, and salt stress conditions. The expression analysis verified by qRT-PCR revealed that IbHQT genes were highly expressed in the HC variety leaves than in the LC variety. Furthermore, cloning and gene function analysis unveiled that IbHQT family genes are involved in the biosynthesis and accumulation of CGA in sweet-potato. This study expands our understanding of the regulatory role of HQT genes in sweet-potato and lays a foundation for further functional characterization and genetic breeding by engineering targeted HQT candidate genes in various sweet-potato varieties and other species.

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