DNA Barcoding Is a Useful Tool for the Identification of the Family Scaridae in Hainan

文献类型： 外文期刊

第一作者： Liu, Bo

作者： Liu, Bo;Yan, Yali;Zhang, Nan;Guo, Huayang;Liu, Baosuo;Yang, Jingwen;Zhu, Kecheng;Zhang, Dianchang;Liu, Bo;Zhang, Nan;Guo, Huayang;Liu, Baosuo;Zhu, Kecheng;Zhang, Dianchang;Zhang, Dianchang;Zhang, Dianchang

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关键词： Scaridae; DNA barcoding; cytochrome c oxidase subunit I (CO I)

期刊名称：JOURNAL OF MARINE SCIENCE AND ENGINEERING （ 影响因子：2.744； 五年影响因子：2.727 ）

ISSN：

年卷期： 2022 年 10 卷 12 期

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收录情况： SCI

摘要： Species markers can be quickly and accurately assessed using DNA barcoding. We investigated samples from the parrotfish family Scaridae using DNA barcoding in Hainan. A total of 401 DNA barcodes were analyzed, including 51 new barcodes generated from fresh material, based on a 533 bp fragment of the cytochrome c oxidase subunit I (CO I) gene. There were 350 CO I barcode clusters that matched 43 species from the Barcode of Life Data Systems (BOLD) and GenBank databases. The results showed the following average nucleotide compositions for the complete dataset: adenine (A, 22.7%), thymine (T, 29.5%), cytosine (C, 29.5%), and guanine (G, 18.2%). The mean genetic distance between confamilial species was nearly 53-fold greater than that between individuals within the species. In the neighbor-joining tree of CO I sequences, Chlorurus sordidus and C. spilurus clustered together, and all other individuals clustered by species. Our results indicated that DNA barcoding could be used as an effective molecular tool for monitoring, protecting, and managing fisheries, and for elucidating taxonomic problem areas that require further investigation.

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