sRNA STnc3020 contributes to the virulence of Salmonella typhimurium may via modulating the gene expression of prgJ of T3SS needle complex

文献类型： 外文期刊

第一作者： Ma, Zhongmei

作者： Ma, Zhongmei;Wei, Lixiang;Wang, Zhanpeng;Li, Nengxiu;Jiao, Jian;Zuo, Yufei;Meng, Qingling;Qiao, Jun;Liu, Yucheng;Xia, Xianzhu;Cai, Xuepeng

作者机构：

关键词： Salmonella typhimurium; Virulence; sRNA STnc3020

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 292 卷

页码：

收录情况： SCI

摘要： As important post-transcriptional regulators of gene expression, sRNAs play important modulatory roles in the environmental adaptation and virulence of bacteria. To investigate the regulatory role of sRNA STnc3020 in the virulence of Salmonella typhimurium (S. typhimurium). This study analyzed the impacts of STnc3020 deletion on adherence, invasion, intracellular survival, macrophage apoptosis, and pathogenicity of S. typhimurium in mice. Furthermore, potential regulatory target genes of STnc3020 were identified and its regulatory mechanism was validated. The results showed that at the cellular level, the deletion of STnc3020 significantly reduced the adhesion ability of S. typhimurium to intestinal epithelial cells (P < 0.01), as well as its proliferation and apoptosis-inducing abilities within macrophages (P < 0.01). Meanwhile, animal experiment results indicated that the deletion of STnc3020 significantly reduced the colonization rate of S. typhimurium in the liver and cecum of mice (P < 0.01), and increased the median lethal dose (4.28 x 10(5)) in mice. Regulatory mechanism research results showed that STnc3020 can interact with the target gene prgJ of the Type III secretion system (T3SS), and the protein level of PrgJ significantly decreased after the deletion of STnc3020 (P < 0.01). These findings offer new insights into sRNA-mediated virulence control and may aid in developing new vaccines and drugs for S. typhimurium.

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