Single-Nucleus RNA Sequencing Reveals the Transcriptome Profiling of Ovarian Cells in Adolescent Cyprinus carpio

文献类型： 外文期刊

第一作者： Hou, Mingxi

作者： Hou, Mingxi;Zhang, Jin;Wang, Qi;Zhao, Ran;Cao, Yiming;Ding, Ning;Sun, Xiaoqing;Zhang, Yan;Li, Jiongtang;Chen, Yingjie;Wang, Kaikuo;Qi, Yingjie;Ding, Ning

作者机构：

关键词： common carp; single-nucleus sequencing; gene expression; oogenesis; granulosa cell

期刊名称：ANIMALS （ 影响因子：2.7； 五年影响因子：3.2 ）

ISSN： 2076-2615

年卷期： 2024 年 14 卷 22 期

页码：

收录情况： SCI

摘要： Simple Summary: The common carp (Cyprinus carpio) shows pronounced sexual dimorphism in growth, and females grow faster than males. Studying the molecular mechanisms of ovarian development and oogenesis is vital for improving productivity and breeding all-female populations. Although many transcriptome analyses of bulk ovaries have been conducted, they have not been able to uncover cell type-specific differences in gene expression. In this study, we presented the transcriptional profile of common carp ovaries at the single-cell level using single-nucleus RNA sequencing. Our analysis revealed valuable information about regulating oogenesis and the development of granulosa cells. Our research provides a helpful resource that complements previously published bulk ovary transcriptomic analyses in the common carp for further investigating the molecular and cellular mechanisms underlying ovarian development.
The common carp (Cyprinus carpio) is a crucial freshwater species cultivated worldwide for food consumption. Female carp have better growth performance than males, which fascinates scholars to uncover the mechanism of gonadal differentiation and produce mono-sex populations. However, the mechanism of ovarian development at single-cell resolution is limited. Here, we conducted single-nucleus RNA sequencing in adolescent common carp ovaries. Our study obtained transcriptional profiles of 13,155 nuclei and revealed 13 distinct cell clusters in the ovaries, including three subtypes of germ cells and four subtypes of granulosa cells. We subsequently performed pseudotime trajectory analysis to delineate potential mechanisms underlying the development of germ cells and granulosa cells. We identified 1250 dynamic expression genes in germ cells and 1815 in granulosa cells (q-value < 0.01), including zp3, eif4a2 and aspm in germ cells and fshr and esr1 in granulosa cells. The functional annotation showed that dynamic expression genes in germ cells were involved in sperm-egg recognition and some terms related to meiosis, such as sister chromatid segregation and homologous recombination. Genes expressed dynamically in granulosa cells were related to the TGF-beta signaling pathway, response to gonadotropin, and development of primary female sexual characteristics. In addition, the dynamic genes expressed in granulosa cells might relate to the complex communication between different cell types. In summary, our study provided a transcriptome profile of common carp ovaries at single-nucleus resolution, and we further revealed the potential cell type-specific mechanisms underlying oogenesis and the differentiation of granulosa cells, which will facilitate breeding all-female common carp populations.

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