Arabidopsis G-Protein beta Subunit AGB1 Negatively Regulates DNA Binding of MYB62, a Suppressor in the Gibberellin Pathway

文献类型： 外文期刊

第一作者： Qi, Xin

作者： Qi, Xin;Tang, Wensi;He, Zhang;Xu, Weiya;Zhou, Yongbin;Wang, Chunxiao;Xu, Zhaoshi;Chen, Jun;Ma, Youzhi;Chen, Ming;Qi, Xin;Fan, Zhijin;Li, Weiwei;Gao, Shiqin

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关键词： Arabidopsis; GA signaling; AGB1; MYB62; protein interaction

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.924； 五年影响因子：6.132 ）

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年卷期： 2021 年 22 卷 15 期

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收录情况： SCI

摘要： Plant G proteins are versatile components of transmembrane signaling transduction pathways. The deficient mutant of heterotrimeric G protein leads to defects in plant growth and development, suggesting that it regulates the GA pathway in Arabidopsis. However, the molecular mechanism of G protein regulation of the GA pathway is not understood in plants. In this study, two G protein beta subunit (AGB1) mutants, agb1-2 and N692967, were dwarfed after exogenous application of GA(3). AGB1 interacts with the DNA-binding domain MYB62, a GA pathway suppressor. Transgenic plants were obtained through overexpression of MYB62 in two backgrounds including the wild-type (MYB62/WT Col-0) and agb1 mutants (MYB62/agb1) in Arabidopsis. Genetic analysis showed that under GA(3) treatment, the height of the transgenic plants MYB62/WT and MYB62/agb1 was lower than that of WT. The height of MYB62/agb1 plants was closer to MYB62/WT plants and higher than that of mutants agb1-2 and N692967, suggesting that MYB62 is downstream of AGB1 in the GA pathway. qRT-PCR and competitive DNA binding assays indicated that MYB62 can bind MYB elements in the promoter of GA2ox7, a GA degradation gene, to activate GA2ox7 transcription. AGB1 affected binding of MYB62 on the promoter of GA2ox7, thereby negatively regulating th eactivity of MYB62.

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