Anti-inflammatory and antioxidant properties of rice bran oil extract in copper sulfate-induced inflammation in zebrafish (Danio rerio)

文献类型： 外文期刊

第一作者： Liu, Naicheng

作者： Liu, Naicheng;Zhang, Peng;Xue, Mingyang;Zhang, Mengjie;Xiao, Zidong;Xu, Chen;Fan, Yuding;Liu, Wei;Zhou, Yong;Liu, Naicheng;Zhang, Peng;Zhang, Mengjie;Zhang, Qinghua;Liu, Naicheng;Zhang, Peng;Zhang, Mengjie;Zhang, Qinghua;Wu, Yeying;Wu, Minglin

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关键词： Anti-inflammatory; Antioxidation; CuSO4; Rice bran oil; Tocotrienols; Zebrafish

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2023 年 136 卷

页码：

收录情况： SCI

摘要： Tocotrienols have strong antioxidant properties; however, tocotrienol has not been investigated in detail in aquatic products. In this study, the anti-inflammatory and antioxidant activities of the tocotrienol-rich fraction from rice bran oil and its potential mechanism were verified in a zebrafish CuSO4 inflammation model. The in vitro antioxidant activity was evaluated using the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) stable radical method. The copper chelating activity was determined using the pyrocatechol violet method. Intracellular reactive ox-ygen species in zebrafish were detected using a fluorescent ROS probe. Transgenic Tg (lyz: DsRed2) zebrafish were used for neutrophil transmigration assays. The mRNA expression levels of antioxidant and pro -inflammatory factor genes were measured using quantitative real-time reverse transcription PCR. In the con-centration range tested, 100 mu g/mL TRF had the highest copper chelating activity (10%). TRF showed DPPH-free radical scavenging ability, which was 53% at 100 mu g/mL TRF. TRF effectively repressed ROS generation and inhibited neutrophil migration to the inflamed site. Moreover, TRF upregulated the expression of antioxidant genes sod and gpx4b, inhibited the expression of pro-inflammatory factors tnfa and il8, and suppressed CuSO4- induced inflammation. In conclusion, TRF has significant anti-inflammatory and antioxidant properties, which supports the use of TRF as an aquatic feed additive to improve the anti-inflammatory and antioxidant capacity of aquatic products.

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