A thioredoxin peroxidase protects Pieris rapae from oxidative stress induced by chlorantraniliprole exposure

文献类型： 外文期刊

第一作者： Zhao, Le

作者： Zhao, Le;Cao, Ye;Wang, Dong-Dong;Chen, Nan;Li, Shi-Guang;Liu, Su;Li, Mao-Ye;Cao, Ye

作者机构：

关键词： antioxidant defense; chlorantraniliprole; Pieris rapae; thioredoxin peroxidase

期刊名称：ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：2.454； 五年影响因子：2.11 ）

ISSN： 0739-4462

年卷期： 2022 年 111 卷 4 期

页码：

收录情况： SCI

摘要： Chlorantraniliprole (CAP) is an insecticide widely used to control the small white butterfly (SWB), Pieris rapae. Exposure to CAP can cause oxidative injury in SWB; however, it is unclear if antioxidant enzymes are involved in the defense process. In this study, a thioredoxin peroxidase (PrTPX1) gene was identified from SWB by using a homology search method. The gene encoded a 195 amino-acid PrTPX1 protein. Sequence characteristics and phylogenetic analysis indicated that PrTPX1 was a typical "2-Cys" TPX, and the PrTPX1 gene consisted of four exons and three introns. Reverse transcription-quantitative polymerase chain reaction analysis indicated that the messenger RNA levels of PrTPX1 were highest in third-, fourth- and fifth-instar larval stages and in the larval midgut. Treatment with sublethal doses (LD20 and LD50) of CAP for 6, 12, 18, and 24 h resulted in increased H2O2 concentration in SWB larvae, indicating insecticide-induced oxidative stress. The transcriptional levels of PrTPX1 were significantly enhanced in larvae exposed to CAP. Recombinant PrTPX1 protein was expressed in Escherichia coli. Enzymatic assay revealed that the protein displayed antioxidant activity and was able to protect against oxidative challenge. These results indicated that PrTPX1 plays an important role in oxidative stress responses and may contribute to the CAP tolerance in SWB.

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