Mapping Molecular Signatures in Plant Leaves, Flowers, and Fruits by a TiO2 Nanotube-Based Plasmonic Chip for Imprint Mass Spectrometry Imaging

文献类型： 外文期刊

第一作者： Chen, Dong

作者： Chen, Dong;Xu, Yizhu;Huang, Yudi;Chen, Yingying;Zhao, Yuhui;Xu, Hanhong;Wu, Xinzhou;Chen, Dong;Xu, Yizhu;Huang, Yudi;Chen, Yingying;Zhao, Yuhui;Xu, Hanhong;Wu, Xinzhou;Yan, Bin;Zhu, Xinhai;Chen, Zilong;Yin, Zhibin

作者机构：

关键词： mass spectrometry imaging; imprinting imaging; SALDI; plasmonic TiO2 nanotube; plant leaves; flowers

期刊名称：ACS AGRICULTURAL SCIENCE & TECHNOLOGY （ 影响因子：2.5； 五年影响因子：2.5 ）

ISSN：

年卷期： 2023 年 3 卷 1 期

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收录情况： SCI

摘要： Mapping molecular signatures within a fragile leaf or flower of medicinal plants with mass spectrometry imaging (MSI) techniques is crucial in plant biology yet remains a great challenge due to the inability to slice such samples. Herein, we constructed a composite substrate, hydrophobic polydopamine-modified TiO2 nanotube coated with plasmonic gold nanoparticle (AuNP-hPDA-TDNT) and exploited its potential as a surface-assisted laser desorption/ionization (SALDI) substrate for imprinting/stamping MSI analysis. With the integrated advantages of satisfactory imprinting/stamping capability and enhanced ion production, this AuNP-hPDA-TDNT substrate-based SALDI-MSI exhibits remarkable performance in mapping tissue-specific distribution patterns of functional metabolites in medicinal plant (i.e., Catharanthus roseus, Ginkgo biloba, and Jatropha integerrima) leaves/flowers and fruits (i.e., strawberry and cherry tomato) regardless of tedious sample preparation. Collectively, we believe that this functionalized TiO2 nanotube substrate will expand the scope of SALDI-MSI in plant science, providing more biologically relevant insights into the functions and mechanisms of plant systems.

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