Actinomadura harenae sp. nov., a novel actinomycete isolated from sea sand in Sanya

文献类型： 外文期刊

第一作者： Hu, Jiangmeihui

作者： Hu, Jiangmeihui;Han, Chuanyu;Yu, Bing;Zhao, Junwei;Guo, Xiaowei;Shen, Yue;Wang, Xiangjing;Xiang, Wensheng;Xiang, Wensheng

作者机构：

关键词： Actinomadura harenae sp. nov.; polyphasic taxonomy; 16S rRNA gene

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2020 年 70 卷 2 期

页码：

收录情况： SCI

摘要： A novel actinomycete, designated strain NEA,U-Ht49(T), was isolated from sea sand sampled in Sanya and characterized by using a polyphasic approach. The 16S rRNA gene sequence analysis showed that strain NEAU-Ht49(T) was most closely related to Actinomadura rhizosphaerae SDA37(T) (98.8%), Actinomadura logoneensis NEAU-G17(T) (98.6%), Actinomadura oligospora ATCC 43269(T) (98.6%) and Actinomadura gamaensis NEAU-Gz5(T) (98.6%). The results of phylogenetic analysis based on the 165 rRNA gene sequences indicated that strain NEAU-Ht49(T) formed a cluster with A. rhizosphaerae SDA37(T), A. logoneensis NEAU-G17(T), A. oligospora ATCC 43269(T), A. gamaensis NEAU-Gz5(T) and Actinomadura rupiterrae CS5-AC15(T) (96.4%). Meso-diaminopimelic acid was detected in its cell walls and glucose, madurose, mannose and ribose were detected in whole-cell hydrolysate. The polar lipids were found to consist of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, phosphatidylinositolmannoside and two unidentified lipids. The majoy menaquinone was MK-10(H-6) and the minor menaquinones were MK-9(H-4) and MK-9(H-8). The major fatty acids were C-16:0, C-18:1 omega 9c, 10-methyl C-18:(0) and iso-C-1(6:)0. Moreover, morphological and chemotaxonomic characteristics of properties of strain NEAU-Ht49(T) also confirmed the affiliation of the isolate to the genus Actinomadura. However, DNA-DNA relatedness, physiological and biochemical data showed that strain NEAU-Ht49(T) could be distinguished from its closest relatives. Therefore, strain NEAU-Ht49(T) represents a novel species of the genus Actinomadura, for which the name Actinomadura harenae sp. nov. is proposed, with strain NEAU-Ht49(T) (=CGMCC 4.7499(T)=JCM 32659(T)) as the type strain.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序