Evaluation of the spinal effects of phthalates in a zebrafish embryo assay

文献类型： 外文期刊

第一作者： Qian, Le

作者： Qian, Le;Liu, Jia;Yuan, Lilai;Shen, Gongming;Yang, Wenbo;Huang, Ying;Mu, Xiyan;Li, Yingren;Qian, Le;Liu, Jia;Chen, Xiaofeng;Pang, Sen;Wang, Chengju;Lin, Zhipeng;Wang, Donghui

作者机构：

关键词： Phthalates; Zebrafish embryo; Spinal effects; Behavioral effect

期刊名称：CHEMOSPHERE （ 影响因子：7.086； 五年影响因子：6.956 ）

ISSN： 0045-6535

年卷期： 2020 年 249 卷

页码：

收录情况： SCI

摘要： Phthalates (phthalate esters, PAEs) are commonly used as plasticizers and are emerging concerns worldwide for their potential influence on the environment and general public health. Thus, identification of the negative effects and involved mechanisms of PAEs is necessary. Herein, we found that embryonic exposure of zebrafish to di-(2-ethylhexyl) phthalate (DEHP) and di-butyl phthalate (DBP) significantly induced spinal defects, such as inhibited spontaneous movement at 24 h post-fertilization (hpf), spine curvature and body length decrease at 96 hpf. The transcriptional level of the genes that are related to the development of the notochord (col8a1a and ngs), muscle (stac3, klhl41a and smyd2b) and skeleton (bmp2, spp1) were significantly altered by DEHP and DBP at 50 and 250 mu g/L, which might be associated with the observed morphological changes. Notably, DBP and DEHP altered the locomotor activity of zebrafish larvae at 144 hpf, which might be due to the abnormal development of the spine and skeletal system. In conclusion, phthalates caused spinal birth defects in zebrafish embryos, induced transcriptional alterations of the spinal developmental genes, and led to abnormal behavior. (C) 2020 Elsevier Ltd. All rights reserved.

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