Characterization and pathogenicity of extracellular serine protease MAP3292c from Mycobacterium avium subsp. paratuberculosis
文献类型： 外文期刊
第一作者： Liu, Hongxiu
作者： Liu, Hongxiu;Dang, Guanghui;Zang, Xinxin;Cai, Zhuming;Cui, Ziyin;Song, Ningning;Liu, Siguo
作者机构：
关键词： Mycobacterium avium subsp. paratuberculosis; MAP3292c; Serine protease; Mycobacterium smegmatis; Pathogenicity
期刊名称：MICROBIAL PATHOGENESIS （ 影响因子：3.738； 五年影响因子：3.663 ）
ISSN： 0882-4010
年卷期： 2020 年 142 卷
页码：
收录情况： SCI
摘要： Serine protease is the virulence factor of many pathogens. However, there are no prevailing data available for serine protease as a virulence factor derived from Mycobacterium avium subsp. paratuberculosis (MAP). The MAP3292c gene from MAP, the predicted serine protease, was expressed in Escherichia coli and characterized by biochemical methods. MAP3292c protein efficiently hydrolyzed casein at optimal temperature and pH of 41 degrees C and 9.0, respectively. Furthermore, divalent metal ions of Ca2+ significantly promoted the protease activity of MAP3292c, and MAP3292c had autocleavage activity between serine 86 and asparagine 87. Site-directed mutagenesis studies showed that the serine 238 residue had catalytic roles in MAP3292c. Furthermore, a BALB/c mouse model confirmed that MAP3292c significantly promoted the survival of Mycobacterium smegmatis in vivo; caused damage to the liver, spleen, and lung; and promoted the release of inflammatory cytokines IL-beta, IL-6, and TNF-alpha in mice. Finally, we confirmed that MAP3292c was relevant to mycobacterial pathogenicity.
分类号：
- 相关文献
作者其他论文 更多>>
-
Modeling praseodymium toxicity in solution to wheat root elongation using the biotic ligand model theory
作者：Guo, Xiaohong;Li, Mengjia;Zong, Haiying;Wang, Fangli;Liu, Shuaiqi;Song, Ningning;Bin, Wang;Huang, Xiaoli;Meng, Li
关键词：Praseodymium; Wheat root elongation; Toxicity; Biotic ligand model (BLM)
-
Five-year controlled-release/stable nitrogen fertilization reduces field nitrogen loss without increasing carbon dioxide emissions in a vegetable rotation system
作者：Wang, Fangli;Li, Zhi;Yu, Zihan;Liu, Jun;Zong, Haiying;Song, Ningning;Zheng, Dan;Liao, Shangqiang;Wang, Xuexia;Zhang, Xi
关键词：controlled-release N fertilizer; greenhouse gas emissions; nitrification inhibitor; stable N fertilizer; vegetable yield
-
Salmonella YrbD protein mediates invasion into the host by interacting with β2 integrin
作者：Cao, Jun;Wang, Jinliang;Dang, Guanghui;Ding, Shihao;Lu, Yao;Xu, Qiu;Liu, Siguo;Yu, Shenye
关键词：132 integrin; adhesion; internalization; Salmonella; protein YrbD
-
XthA phospholipase contributes the cell-wall functionality of pathogenic mycobacteria by increasing the synthesis of mycolic acid
作者：Jiang, Yanyan;Zang, Xinxin;Feng, Tingting;Wang, Hui;Cui, Ning;Duan, Yifan;Chen, Chunwen;Zhang, Zhenjun;Guo, Ruonan;Gao, Yunjie;Cui, Yingying;Dang, Guanghui;Liu, Siguo;Zang, Xinxin;Zang, Xinxin;Dang, Guanghui
关键词：Mycobacterial phospholipase; Cell wall; Mycolic acid
-
Serine protease Rv2569c inhibits inflammatory response and promotes intracellular survival of Mycobacterium tuberculosis by targeting the RhoG-NF-κB-NLRP3 pathway
作者：Zang, Xinxin;Zhang, Jiajun;Feng, Tingting;Wang, Hui;Cui, Yingying;Jiang, Yanyan;Chen, Chunwen;Liu, Siguo;Dang, Guanghui
关键词：Mycobacterium tuberculosis Rv2569c; NLRP3 inflammasome; NF-kappa B signaling pathway
-
Characterization of an enterococcal phage endolysin as a potential antimicrobial agent against Streptococcus suis
作者：Wang, Jing;Liu, Yaowei;Liang, Siyu;Lu, Xiaofeng;Xu, Qiu;Fan, Cuilong;Zhang, Wanjiang;Liu, Siguo;Xie, Fang
关键词：
Streptococcus suis ; phage; endolysin; antibacterial activity; anti-biofilm activity -
Fe-CGS Effectively Inhibits the Dynamic Migration and Transformation of Cadmium and Arsenic in Soil
作者：Yin, Hongliang;Song, Ningning;Song, Xin;Zong, Quanli;Yin, Hongliang;Zhou, Changzhi;Wang, Junhuan;Yin, Mengxue;Wu, Zhihao;Hou, Hong;Shangguan, Yuxian;Sun, Zaijin
关键词：iron modification; coal gasification slag; cadmium; arsenic; leaching; stabilization
