文献类型： 外文期刊
第一作者： Shen, X. H.
作者： Shen, X. H.;Zheng, W.;Feng, P.;Lai, Y. C.;Shen, X. H.;Jiang, C.;Wang, Q.
作者机构：
关键词： Co-60-gamma irradiation; ethyl methane sulfonate; seedling growth; antioxidant enzyme activity; chlorophyll content
期刊名称：APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH （ 影响因子：0.711； 五年影响因子：0.796 ）
ISSN： 1589-1623
年卷期： 2020 年 18 卷 2 期
页码：
收录情况： SCI
摘要： In order to study the effect of mutagenesis on growth, physiology and cell structure in alfalfa (Medicago sativa L), the seeds of Longmu806 were treated with two kinds of mutagenesis methods including Co-60-gamma irradiation (50, 150, 300, 450, 600 Gy) and EMS (ethyl methane sulfonate 0.1%, 0.2%, 0.4% (v/v)). The results showed that the seed germination rate, seedling growth, fresh grass yield and plant height were significantly (P < 0.05) higher than their corresponding controls under 50-150 Gy, and then decreased with the increasing of radiation dose. However, these indicators decreased with the increasing of EMS concentration. Two mutagenic treatments increased alfalfa branch number. The chlorophyll content was promoted by a dose of 50-150 Gy and 0.4% EMS concentration. It was also found that mutagenic treatments could promote the activity of three anti-oxidant enzymes including superoxide dismutase (SOD), peroxidase (POD) and hydrogen peroxidase (CAT), and the highest antioxidant enzyme activity was 150 Gy Co-60-gamma irradiation and 0.4% EMS treatment. The leaf thicknesses increased, the vein protuberant degrees decreased and the compactness of cell structure was enhanced. Being more conducive for screening mutants, Co-60-gamma irradiation with 150 Gy and EMS with 0.4% were the ideal mutagenic treatments.
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