The immune response to a recombinant Lactococcus lactis oral vaccine against foot-and-mouth disease virus in mice

文献类型： 外文期刊

第一作者： Liu, Xinsheng

作者： Liu, Xinsheng;Qi, Linlin;Lv, Jianliang;Zhang, Zhongwang;Zhou, Peng;Ma, Zhongyuan;Wang, Yonglu;Zhang, Yongguang;Pan, Li;Zhang, Yongguang;Pan, Li

作者机构：

关键词： Lactococcus lactis; Oral vaccine; Mucosal immunity; Foot-and-mouth disease virus; VP1 protein

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

ISSN： 0141-5492

年卷期： 2020 年 42 卷 10 期

页码：

收录情况： SCI

摘要： Objective Development of an effective mucosal vaccine to induce specific immune responses against Foot-and-mouth disease virus (FMDV). Results For this purpose, the FMDV VP1 gene (SPVP1) was optimized and synthesized based on the codon bias of Lactococcus lactis (L. lactis), and then incorporated in the plasmid pNZ8148. L. lactis NZ9000 containing the pNZ8148-SPVP1 recombinant plasmid was used as an oral delivery vehicle to induce anti-FMDV mucosal and systemic immune responses in mice. After confirmation that the SPVP1 protein was expressed successfully in the recombinant L. latic, the mice were orally challenged with NZ9000-pNZ8148, NZ9000-pNZ8148-SPVP1, phosphate-buffered saline as a mock infection group, or with inactivated vaccine as a positive group. Mice immunized with NZ9000-pNZ8148-SPVP1 produced high levels of mucosal secretory IgA (sIgA), antigen-specific serum IgG, IgA, and neutralizing antibodies, and developed stronger cell-mediated immune reactions and significant T spleen lymphocyte proliferation. Furthermore, the recombinant group generated much higher levels of IFN-gamma, IL-2, IL-4, IL-5, and IL-10 than the other groups. Conclusions Potent immune responses were successfully elicited in mice with FMDV VP1 delivered through L. lactis.

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