Lactobacillus plantarum FRT10 alleviated high-fat diet-induced obesity in mice through regulating the PPAR alpha signal pathway and gut microbiota

文献类型： 外文期刊

第一作者： Cai, Hongying

作者： Cai, Hongying;Wen, Zhiguo;Li, Xiumei;Meng, Kun;Yang, Peilong;Cai, Hongying;Yang, Peilong

作者机构：

关键词： Lactobacillus plantarum FRT10; Sour dough; Obesity; Gut microbiota; PPAR alpha

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2020 年 104 卷 13 期

页码：

收录情况： SCI

摘要： Previous studies showed that probiotics supplementation contributed to alleviate obesity. This work was to assess the efficacy of Lactobacillus plantarum FRT10 from sour dough in alleviating obesity in mice fed with a high-fat diet (HFD), and the underlying mechanisms focusing on modulation of the gut microbiota profile. Kunming mice were fed with a regular diet (CT), a high-fat diet (HFD), and two HFDs containing low and high doses of L. plantarum FRT10 for 8 weeks. The physiological and biochemical modulations in liver were analyzed. Cecal contents were analyzed by high-throughput 16S ribosomal RNA sequencing. FRT10 supplementation significantly reduced body weight gain, fat weight, and liver triacylglycerols (TGs) and alanine aminotransferase (ALT) concentrations (P < 0.05). FRT10 significantly ameliorated the HFD-induced gut dysbiosis, as evidenced by increased abundance of microbes, including Butyricicoccus, Butyricimonas, Intestinimonas, Odoribacter, and Alistipes, and decreased abundance of Desulfovibrionaceae, Roseburia, and Lachnoclostridium. Lactobacillus, Bifidobacterium, and Akkermansia were markedly increased after FRT10 intervention. In addition, real-time quantitative PCR revealed that FRT10 upregulated the mRNA expression levels of peroxisome proliferator-activated receptor-alpha (PPAR alpha) and carnitine palmitoyltransferase-1 alpha (CPT1 alpha), and downregulated the mRNA expression levels of sterol regulatory element-binding protein 1 (SREBP-1) and TG-synthesizing enzyme diacylglycerol acyltransferase 1 (DGAT1) in liver. These findings suggested that FRT10 had anti-obesity effects in obese mice partly related to the activation of PPAR alpha/CPT1 alpha pathway. FRT10 can be considered a single probiotic agent for preventing HFD-induced obesity in humans and animals.

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