Rapid and efficient detection methods of pathogenic swine enteric coronaviruses

文献类型： 外文期刊

第一作者： Fu, Yuguang

作者： Fu, Yuguang;Li, Baoyu;Liu, Guangliang

作者机构：

关键词： RT-PCR; detection methods; Swine Diarrhea; Pathogenic enteric coronaviruses

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：4.813； 五年影响因子：4.697 ）

ISSN： 0175-7598

年卷期： 2020 年 104 卷 14 期

页码：

收录情况： SCI

摘要： Porcine enteric coronaviruses (CoVs) cause highly contagious enteric diarrhea in suckling piglets. These COV infections are characterized by clinical signs of vomiting, watery diarrhea, dehydration, and high morbidity and mortality, resulting in significant economic losses and tremendous threats to the pig farming industry worldwide. Because the clinical manifestations of pigs infected by different CoVs are similar, it is difficult to differentiate between the specific pathogens. Effective high-throughput detection methods are powerful tools used in the prevention and control of diseases. The immune system of piglets is not well developed, so serological methods to detect antibodies against these viruses are not suitable for rapid and early detection. This paper reviews various PCR-based methods used for the rapid and efficient detection of these pathogenic CoVs in swine intestines.

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