Regioselective sulfation of beta-glucan from Ganoderma lucidum and structure-anticoagulant activity relationship of sulfated derivatives

文献类型： 外文期刊

第一作者： Zhang, Zhong

作者： Zhang, Zhong;Tang, Qingjiu;Wu, Di;Zhou, Shuai;Yang, Yan;Feng, Na;Tang, Chuanhong;Wang, Jinyan;Yan, Mengqiu;Liu, Yanfang;Zhang, Jingsong

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关键词： Sulfated beta-glucan; Regioselective synthesis; Structure-activity relationship

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2020 年 155 卷

页码：

收录情况： SCI

摘要： In the present study, regioselective sulfation of beta-glucan (GLP) from Ganoderma lucidum were firstly established by using 4,4'-dimethoxytrityl chloride and hexamethyldisilazane as protecting precursor. 2,4,6-O-sulfated, 6-O-sulfated and 2,4-O-sulfated GLP derivatives were prepared and the molecular weights (M-w) of derivatives were determined to range from 0.94 x 10(4) to 6.27 x 10(4) g/mol, while the degrees of sulfation (DS) were calculated to vary from 0.83 to 1.74. The regioselective sulfation of GLP was confirmed by FT-IR, C-13 NMR spectroscopy and methylation analysis. Results indicated that the sulfated substitution sites were predominantly at C-6 in 6-O-sulfated GLP (S(6-O)GLP) and C-4 in 2,4-O-sulfated GLP (S(2,4-O)GLP), respectively. Clotting assays (APTT, PT and TT) in vitro showed that sulfate groups were essential for anticoagulant activity and S(6-O)GLP exhibited much higher than others. Meanwhile, sulfated GLP with higher DS and M-w showed stronger anticoagulant activity in the case of the same condition. (C) 2020 Elsevier B.V. All rights reserved.

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