Pantothenic acid promotes dermal papilla cell proliferation in hair follicles of American minks via inhibitor of DNA Binding 3/Notch signaling pathway

文献类型： 外文期刊

第一作者： Wang, Zhuo

作者： Wang, Zhuo;Nan, Weixiao;Si, Huazhe;Li, Guangyu;Zhang, Haihua;Wang, Shiyong

作者机构：

关键词： Pantothenic acid; ID3; Notch; Hair follicle; Dermal papilla cell

期刊名称：LIFE SCIENCES （ 影响因子：5.037； 五年影响因子：4.689 ）

ISSN： 0024-3205

年卷期： 2020 年 252 卷

页码：

收录情况： SCI

摘要： Aims: Pantothenic acid (PA) has been applied to treat alopecia, but the underlying mechanism is still unclear. Our study aims to explore the underlying mechanism of PA in regulating hair follicle (HF) growth. Main methods: Mink HFs and dermal papilla (DP) cells were isolated and cultured in vitro. HFs and DP cells were treated with 0, 10, 20, 40 mu g/ml PA. The effect of PA on HF growth, DP cell proliferation, cell cycle distribution, cell migration, and insulin-like growth factor-1 (IGF-1) and vascular endothelial growth factor (VEGF) expressions in DP cells was measured. Moreover, the effect of PA on inhibitor of DNA binding 3 (ID3)/Notch signaling pathway was analyzed. Subsequently, ID3 was silenced to validate whether ID3/Notch signaling pathway was involved in regulating DP cell proliferation by PA. Key findings: Both 20 mu g/ml and 40 mu g/ml PA promoted HF growth, G1/S transition of DP cells and IGF-1 and VEGF expressions in DP cells, while only 20 mu g/ml PA promoted cell viability and the migration of DP cells. Thus 20 mu g/ml PA was chosen for the following experiments. PA treatment was found to up-regulate ID3 expression but down-regulate Notch receptor 1 (Notch1) and Notch signaling targets expressions. Furthermore, ID3 knockdown reversed PA-induced cell proliferation and inhibition of Notch1 and Notch signaling targets expressions, indicating that PA-induced DP cell proliferation and inhibition of Notch signaling were mediated via up-regulation of ID3. Significance: This study provides an underlying mechanism related to the effect of PA on stimulating DP cell proliferation.

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