Stability comparison of three candidate internal reference genes in Chinese giant salamander (Andrias davidianus)

文献类型： 外文期刊

第一作者： Qian, Jing

作者： Qian, Jing;Deng, Dun;Qian, Jing

作者机构：

关键词： Chinese giant salamander (Andrias davidianus); immune challenge; qPCR; reference genes

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

ISSN： 1355-557X

年卷期： 2020 年 51 卷 1 期

页码：

收录情况： SCI

摘要： The Chinese giant salamander (Andrias davidianus) as food and medicinal product has been an important aquaculture object in China. Study of gene function in the Chinese giant salamander requires accurate normalization though the use of appropriate reference genes. In this study, the expression levels of three candidate reference genes including beta-actin, GAPDH and cytb of different tissues, different developmental stages and different challenges in Chinese giant salamander were evaluated by qPCR. The stabilities of these three reference genes were analysed by geNorm, NormFinder and BestKeeper software. The results showed that the expression of GAPDH was more stable than that of beta-actin and cytb in four tissues and at two developmental stages of Chinese giant salamander. Compared with GAPDH and cytb, beta-actin was the most stable in spleen of Chinese giant salamander treated with LPS or GSIV. Therefore, the result showed that GAPDH was the suitable reference gene in different tissues and at different developmental stages of Chinese giant salamander. The beta-actin could be used as a reference gene in spleen of Chinese giant salamander challenged with LPS and GSIV. This study provides convincing information for the GAPDH and beta-actin as suitable reference gene in Chinese giant salamander of different tissues, different developmental stages and different challenges respectively.

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[1]Stability comparison of three candidate internal reference genes in Chinese giant salamander (Andrias davidianus). Qian, Jing,Deng, Dun,Qian, Jing.

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