Flower transcriptome dynamics during nectary development in pepper (Capsicum annuum L.)

文献类型： 外文期刊

第一作者： Deng, Ming-hua

作者： Deng, Ming-hua;Zhao, Kai;Zhu, Hai-shan;Deng, Ming-hua;Lv, Jun-heng;Zhang, Zhu-qing;Zou, Xue-xiao;Deng, Ming-hua;Wen, Jin-fen;Wen, Jin-fen;Huo, Jin-long

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关键词： Capsicum annuum L.; flower transcriptome; nectar biosynthesis; nectary development; gene

期刊名称：GENETICS AND MOLECULAR BIOLOGY （ 影响因子：1.771； 五年影响因子：2.584 ）

ISSN： 1415-4757

年卷期： 2020 年 43 卷 2 期

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收录情况： SCI

摘要： The measurement of gene expression can provide important information about gene function and the molecular basis for developmental processes. We analyzed the transcriptomes at three different developmental stages of pepper flower [sporogenous cell division, stage (B1); pollen mother cell meiosis, stage (B2); and open flower (B3)]. In the cDNA libraries for B1, B2, and B3: 82718, 77061, and 91491 unigenes were assembled, respectively. A total of 34,445 unigene sequences and 128 pathways were annotated by KEGG pathway analysis. Several genes associated with nectar biosynthesis and nectary development were identified, and 8,955, 12,182, and 23,667 DEGs were identified in the B2 vs B1, B3 vs B1, and B3 vs. B2 comparisons. DEGs were involved in various metabolic processes, including flower development, nectar biosynthesis, and nectary development. According to the RNA-seq data, all 13 selected DEGs showed similar expression patterns after q-PCR analysis. Sucrose-phosphatase, galactinol-sucrose galactosyltransferase, and sucrose synthase played very important roles in nectar biosynthesis, and CRABS CLAW could potentially be involved in mediating nectary development. A significant number of simple sequence repeat and single nucleotide polymorphism markers were predicted in the Capsicum annuum sequences. The new results provide valuable genetic information about flower development in pepper.

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