Genome and single-cell RNA-sequencing of the earthworm Eisenia andrei identifies cellular mechanisms underlying regeneration

文献类型： 外文期刊

第一作者： Shao, Yong

作者： Shao, Yong;Zhang, Jin-Jin;Li, Ming-Li;Ma, Xi-Yao;Irwin, David M.;Zhang, Guo-Jie;Wu, Dong-Dong;Wang, Xiao-Bo;Ruan, Jue;Zhang, Jin-Jin;Li, Ming-Li;Wu, Shou-Song;Wang, Xue;Zhao, Hui-Fang;Zhu, Helen He;Wang, Xue;Zhao, Hui-Fang;Zhu, Helen He;Wang, Xue;Zhao, Hui-Fang;Zhu, Helen He;Li, Yuan;Wang, De-Peng;Irwin, David M.;Irwin, David M.;Zhang, Guo-Jie;Zhang, Guo-Jie;Zhang, Guo-Jie;Wu, Dong-Dong

作者机构：

期刊名称：NATURE COMMUNICATIONS （ 影响因子：14.919； 五年影响因子：15.805 ）

ISSN： 2041-1723

年卷期： 2020 年 11 卷 1 期

页码：

收录情况： SCI

摘要： The earthworm is particularly fascinating to biologists because of its strong regenerative capacity. However, many aspects of its regeneration in nature remain elusive. Here we report chromosome-level genome, large-scale transcriptome and single-cell RNA-sequencing data during earthworm (Eisenia andrei) regeneration. We observe expansion of LINE2 transposable elements and gene families functionally related to regeneration (for example, EGFR, epidermal growth factor receptor) particularly for genes exhibiting differential expression during earthworm regeneration. Temporal gene expression trajectories identify transcriptional regulatory factors that are potentially crucial for initiating cell proliferation and differentiation during regeneration. Furthermore, early growth response genes related to regeneration are transcriptionally activated in both the earthworm and planarian. Meanwhile, single-cell RNA-sequencing provides insight into the regenerative process at a cellular level and finds that the largest proportion of cells present during regeneration are stem cells. The mechanisms regulating regeneration of the earthworm are unclear. Here, the authors use genomic and transcriptomic analysis of the earthworm Eisenia andrei together with Hi-C analysis to identify genes involved and show activation of LINE2 transposable elements on regeneration.

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