Improved Integrated Whole Proteomic and Phosphoproteomic Profiles of Severe Acute Pancreatitis

文献类型： 外文期刊

第一作者： Wang, Cheng

作者： Wang, Cheng;Chen, Bicheng;Sun, Linxiao;Zhang, Yanlei;Tan, Jinjuan

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关键词： severe acute pancreatitis; proteomic; phosphoproteomic; DDA; DIA

期刊名称：JOURNAL OF PROTEOME RESEARCH （ 影响因子：4.466； 五年影响因子：4.352 ）

ISSN： 1535-3893

年卷期： 2020 年 19 卷 6 期

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收录情况： SCI

摘要： Severe acute pancreatitis (SAP) is caused by complicated biological factors, and revealing its complex pathogenesis by single-target analysis is difficult. Systematic studies have developed slowly because extraction of degradable pancreatic proteins exposed to multiple proteases is challenging. We present integrated whole proteomic and phosphoproteomic profiles of SAP rats based on a modified protein extraction strategy with less protein degradation. Data-dependent acquisition (DDA) and data-independent acquisition (DIA) strategies were applied to select an appropriate method. Total 275 differentially expressed proteins and 757 differentially expressed phosphorylated proteins were identified by DIA-based quantitative proteomics. Several signal transduction pathways, including the AMPK, MAPK, and PI3K-Akt pathways, were enriched in SAP. Up-regulation of phosphorylated proteins involved in the process of TNFA signaling and inflammatory response was also detected in SAP. Our results improve the understanding of SAP development and progression.

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