HAI-1 regulates placental folds development by influencing trophoblast cell proliferation and invasion in pigs

文献类型： 外文期刊

第一作者： Hong, Linjun

作者： Hong, Linjun;He, Yanjuan;Tan, Chengquan;Wu, Zhenfang;Hong, Linjun;Yu, Mei;Hong, Linjun;He, Yanjuan;Tan, Chengquan;Wu, Zhenfang

作者机构：

关键词： HAI-1; Placenta; Trophoblast cell; Development; Pig

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2020 年 749 卷

页码：

收录情况： SCI

摘要： Fetal development is critically dependent on the efficiency of the placenta. Porcine trophoblast cell proliferation and invasion have crucial roles in placental fold development, which is one of the essential events determining placental efficiency. The membrane serine proteinase inhibitor hepatocyte growth factor activator inhibitor-1 (HAI-1) can regulate cellular invasion and motility in different types of epithelial cells, including trophoblast cells in mice. This work used quantitative polymerase chain reaction (qPCR) and immunohistochemistry to compare the expression level and location of HAI-1 in the placenta on gestational days 26, 50, and 95 in Yorkshire and Meishan pigs. The role of HAI-1 in porcine trophoblast cell (PTr2) proliferation, invasion, and migration in vitro was investigated by analyzing the effects of HAI-1 gene silencing or overexpression. Polymorphism in the HAI-1 gene was detected to determine associations between the genotype and piglet birth weight in 400 healthy pure-bred Yorkshire piglets. qPCR results showed that HAI-1 mRNA levels significantly increased (P < 0.01) between gestational days 26 and 50 and then decreased (P < 0.01) between days 50 and 95 in both Meishan and Yorkshire pigs. Immunohistochemical analysis showed that HAI-1 protein was strongly expressed by the high columnar trophoblast cells located at the top of the placental folds with low proliferative and invasion capacities. However, it was expressed at very low levels in cuboidal trophoblast cells located at the side and base of the placental folds with high proliferative and invasion capacities. In vitro experiments indicated that HAI-1 had the ability to reduce the proliferation, invasion and migration of trophoblast cells. In addition, one single-nucleotide polymorphism (SNP) of HAI-1 showed a significant association (P < 0.05) with piglet birth weight. These results revealed that HAI-1 could be a vital molecule in placental folds development by regulating trophoblast proliferation and invasion in pigs.

分类号：

  • 相关文献
作者其他论文 更多>>
  • Analysis of differential transcriptome expression reveals that ISG15 provides support for embryo development by promoting angiogenesis in porcine mesometrium

    作者：Gan, Jianyu;Wang, Yongzhong;Zhang, Fan;Zang, Xupeng;Gu, Ting;Xu, Zheng;Li, Zicong;Cai, Gengyuan;Wu, Zhenfang;Hong, Linjun;Gan, Jianyu;Wang, Yongzhong;Zhang, Fan;Zang, Xupeng;Gu, Ting;Xu, Zheng;Li, Zicong;Cai, Gengyuan;Wu, Zhenfang;Hong, Linjun;Meng, Fanming;Wu, Zhenfang;Hong, Linjun

    关键词：Pig; Mesometrium; Transcriptome; Interferon response; ISG15

  • GPCPD1 is regulated by METTL3/IGF2BP3 dependent m6A modification to affect pig muscle fiber type conversion

    作者：Yang, Yuming;Xiao, Liyao;Huang, Huijun;Su, Jiawei;Hong, Linjun;Cai, Gengyuan;Li, Zicong;Gu, Ting;Yang, Yuming;Xiao, Liyao;Huang, Huijun;Su, Jiawei;Hong, Linjun;Cai, Gengyuan;Li, Zicong;Gu, Ting;Tan, Baohua;Xu, Weihua;Zhao, Yaolu;Dai, Lihe

    关键词：GPCPD1; Pig; m 6 A; Myogenic differentiation; Muscle fiber type conversion

  • Genome-Wide Association Study Reveals Genetic Mechanisms Underlying Intersex and Aproctia in Large White Pigs

    作者：Li, Yajun;Shi, Jiaxin;Yang, Yingshan;Ruan, Donglin;Wu, Jie;Lin, Danyang;Liao, Zihao;Hong, Xinrun;Zhou, Fuchen;Liu, Langqing;Yang, Jie;Zheng, Enqin;Wu, Zhenfang;Cai, Gengyuan;Zhang, Zebin;Li, Yajun;Shi, Jiaxin;Yang, Yingshan;Ruan, Donglin;Wu, Jie;Lin, Danyang;Liao, Zihao;Hong, Xinrun;Zhou, Fuchen;Liu, Langqing;Yang, Jie;Zheng, Enqin;Wu, Zhenfang;Cai, Gengyuan;Zhang, Zebin;Wu, Jie;Yang, Ming;Wu, Zhenfang;Cai, Gengyuan;Wu, Zhenfang;Cai, Gengyuan

    关键词：intersex; aproctia; chip data; imputed data; mixed linear models; genome-wide association studies

  • Optimizing the Size of Zr-Based Metal-Organic Frameworks for Enhanced Anticancer Efficacy

    作者：Cheng, Zan;Yu, Mei;Zhang, Ruiting;Li, Fangshu;Wang, Shanshan;She, Yongxin;Wan, Yilong;Xiang, Huandong;Wei, Haoran;Zu, Xu;Li, Xin;Wan, Yilong;Xiang, Huandong;Wei, Haoran;Zu, Xu;Li, Xin

    关键词：metal-organic frameworks; UiO-67; drug delivery; cellular internalization; anticancer

  • Regulation of myo-miR-24-3p on the Myogenesis and Fiber Type Transformation of Skeletal Muscle

    作者：Fan, Danyang;Liu, Yanwen;Yu, Mei;Tang, Zhonglin;Fan, Danyang;Liu, Yanwen;Yu, Mei;Tang, Zhonglin;Fan, Danyang;Liu, Yanwen;Yan, Chao;Li, Fanqinyu;Wang, Shilong;Tang, Zhonglin;Fan, Danyang;Yao, Yilong;Liu, Yanwen;Yan, Chao;Li, Fanqinyu;Wang, Shilong;Tang, Zhonglin;Yan, Chao;Wang, Shilong;Tang, Zhonglin;Xie, Bingkun

    关键词：porcine; miR-24-3p; skeletal muscle fiber transformation; regeneration

  • A deep learning-based approach for fully automated segmentation and quantitative analysis of muscle fibers in pig skeletal muscle

    作者：Yao, Zekai;Li, Hao;Li, Xinxin;Li, Jianhao;Luo, Yizhi;Meng, Fanming;Yao, Zekai;Zheng, Enqin;Yang, Jie;Li, Hao;Li, Xinxin;Wang, Ting;Fan, Zhenfei;Zhan, Yuexin;Yang, Yingshan;Wu, Zhenfang;Yao, Zekai;Zheng, Enqin;Yang, Jie;Li, Hao;Li, Xinxin;Wang, Ting;Fan, Zhenfei;Zhan, Yuexin;Yang, Yingshan;Wu, Zhenfang;Wo, Jingjie;Yin, Ling;Wu, Zhenfang;Luo, Yizhi;Zheng, Enqin;Yang, Jie;Wu, Zhenfang

    关键词：Pigs; Skeletal muscle; Deep learning; Image segmentation; Quantitative analysis

  • Multi-omic characterization of allele-specific regulatory variation in hybrid pigs

    作者：Quan, Jianping;Wang, Xingwang;Cai, Gengyuan;Ding, Rongrong;Zhuang, Zhanwei;Zhou, Shenping;Ruan, Donglin;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Meng, Fanming;Wu, Jie;Xu, Cineng;Qiu, Yibin;Wang, Shiyuan;Lin, Meng;Li, Shaoyun;Ye, Yong;Zhou, Fuchen;Lin, Danyang;Li, Xuehua;Deng, Shaoxiong;Zhang, Yuling;Yao, Zekai;Yang, Yingshan;Liu, Yiyi;Zhan, Yuexin;Zhang, Jiaming;Ma, Fucai;Yang, Jifei;Chen, Qiaoer;Yang, Jisheng;Gu, Ting;Huang, Sixiu;Xu, Zheng;Li, Zicong;Yang, Jie;Wu, Zhenfang;Quan, Jianping;Ding, Rongrong;Tan, Suxu;Huang, Wen;Quan, Jianping;Cai, Gengyuan;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Lin, Meng;Huang, Sixiu;Yang, Jie;Quan, Jianping;Ding, Rongrong;Ye, Jian;Dong, Linsong;Wu, Zhenfang;Yang, Ming;Gao, Xin;Liu, Zhihong;Yang, Ming;Ding, Rongrong;Ye, Jian;Dong, Linsong;Wu, Zhenfang;Wang, Xingwang;Cai, Gengyuan;Zhuang, Zhanwei;Zhou, Shenping;Ruan, Donglin;Wu, Jiajin;Zheng, Enqin;Zhang, Zebin;Liu, Langqing;Wu, Jie;Xu, Cineng;Qiu, Yibin;Wang, Shiyuan;Li, Shaoyun;Ye, Yong;Zhou, Fuchen;Lin, Danyang;Li, Xuehua;Deng, Shaoxiong;Zhang, Yuling;Yao, Zekai;Yang, Yingshan;Liu, Yiyi;Zhan, Yuexin;Zhang, Jiaming;Ma, Fucai;Yang, Jifei;Gu, Ting;Xu, Zheng;Li, Zicong;Yang, Jie;Meng, Fanming

    关键词：

微信小程序