Biodegradation of Bisphenol A by Sphingobium sp. YC-JY1 and the Essential Role of Cytochrome P450 Monooxygenase

文献类型： 外文期刊

第一作者： Jia, Yang

作者： Jia, Yang;Eltoukhy, Adel;Wang, Junhuan;Li, Xianjun;Hlaing, Thet Su;Aung, Mar Mar;Nwe, May Thet;Yan, Yanchun;Lamraoui, Imane

作者机构：

关键词： bisphenol A; Sphingobium sp; biodegradation; cytochrome P450; soil remediation

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.923； 五年影响因子：6.132 ）

ISSN：

年卷期： 2020 年 21 卷 10 期

页码：

收录情况： SCI

摘要： Bisphenol A (BPA) is a widespread pollutant threatening the ecosystem and human health. An effective BPA degrader YC-JY1 was isolated and identified as Sphingobium sp. The optimal temperature and pH for the degradation of BPA by strain YC-JY1 were 30 degrees C and 6.5, respectively. The biodegradation pathway was proposed based on the identification of the metabolites. The addition of cytochrome P450 (CYP) inhibitor 1-aminobenzotriazole significantly decreased the degradation of BPA by Sphingobium sp. YC-JY1. Escherichia coli BL21 (DE3) cells harboring pET28a-bisdAB achieved the ability to degrade BPA. The bisdB gene knockout strain YC-JY1 Delta bisdB was unable to degrade BPA indicating that P450(bisdB) was an essential initiator of BPA metabolism in strain YC-JY1. For BPA polluted soil remediation, strain YC-JY1 considerably stimulated biodegradation of BPA associated with the soil microbial community. These results point out that strain YC-JY1 is a promising microbe for BPA removal and possesses great application potential.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序