Characterization of the phosphotriesterase capable of hydrolyzing aryl-organophosphate flame retardants

文献类型： 外文期刊

第一作者： Wang, Junhuan

作者： Wang, Junhuan;Wang, Junhuan;Yuan, Long;Wu, Wei;Yan, Yanchun

作者机构：

关键词： Organophosphate flame retardants; Sphingopyxis; Phosphotriesterase; 2-ethylhexyl diphenyl phosphate; Resorcinol bis(diphenyl phosphate); Horizontal gene transfer

期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：5.56； 五年影响因子：5.365 ）

ISSN： 0175-7598

年卷期： 2022 年 106 卷 19-20 期

页码：

收录情况： SCI

摘要： A related group of phosphotriesters known as organophosphate flame retardants (OPFRs) has become emerging contaminants due to its worldwide use. The lack of an easily hydrolysable bond renders OPFRs inert to the well-known phosphotriesterases capable of hydrolyzing the neurotoxic organophosphates. An OPFRs phosphotriesterase gene stpte was cloned from plasmid pStJH of strain Sphingopyxis terrae subsp. terrae YC-JH3 and was heterologously expressed in Escherichia coli. The recombinant protein St-PTE was purified and analyzed. St-PTE showed the highest catalytic activity at pH 8.5 and 35 degrees C. The optimal substrate for St-PTE is triphenyl phosphate, with k(cat)/K-m of 5.03 x 10(6) M-1 s(-1), two orders of magnitude higher than those of tricresyl phosphate (4.17 x 10(4) M-1 s(-1)), 2-ethylhexyl diphenyl phosphate (2.03 x 10(4) M-1 s(-1)) and resorcinol bis(diphenyl phosphate) (6.30 x 10(4) M-1 s(-1)). St-PTE could break the P-O bond of tri-esters and convert aryl-OPFRs into their corresponding di-ester metabolites, including polymers of resorcinol bis(diphenyl phosphate). Mediated by transposase, the gene of OPFRs phosphotriesterase could be transferred horizontally among closely related strains of Sphingomonas, Sphingobium and Sphingopyxis.

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