Determination of thirteen acidic phytohormones and their analogues in tea (Camellia sinensis) leaves using ultra high performance liquid chromatography tandem mass spectrometry

文献类型： 外文期刊

第一作者： Jiang, Changling

作者： Jiang, Changling;Dai, Jinxia;Han, Haolei;Wang, Chen;Zhu, Li;Lu, Chengyin;Chen, Hongping;Jiang, Changling;Dai, Jinxia;Han, Haolei;Wang, Chen;Zhu, Li;Lu, Chengyin;Chen, Hongping

作者机构：

关键词： Tea; Acidic phytohormones; Ultra high performance liquid chromatography-tandem mass spectrometry; Dormancy; Light qualities

期刊名称：JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES （ 影响因子：3.205； 五年影响因子：3.068 ）

ISSN： 1570-0232

年卷期： 2020 年 1149 卷

页码：

收录情况： SCI

摘要： Trace plant hormones play an important role in tea growth, development and quick response to biotic and abiotic stresses. However, lack of a sensitive method limits the research on plant hormone regulation for tea quality and yields. Herein, a highly sensitive method was developed using ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) for profiling and quantification of 13 acidic phytohormones and their analogues, including auxins, abscisic acid and gibberellins in fresh tea leaves. After optimizing the different C18 columns and mobile phase systematically, an Agilent Eclipse Plus C18 column combined with the mobile phase A (acetonitrile) and B (water) was employed. Target acidic phytohormones were extracted using acidified methanol, and tea matrices were cleaned up by dispersive solid phase adsorbents of polyvinylpolypyrrolidone (PVPP) and graphitized carbon black (GCB) followed by polymer-based mixedmode cation-exchange solid phase extraction. The method showed good linearity for all 13 analytes with regression coefficients (R-2) > 0.998. Satisfactory recoveries of 12 analytes spiked with three levels ranged from 71.8% to 109.9%, while intra-day and inter-day precisions were below 20%. Limits of detection (LODs) and limits of quantitation (LODs) for 12 acidic phytohormones were 0.1-4.2 mu g kg(-1) and 0.3-13.9 mu g kg(-1), respectively. Finally, this method was firstly employed to analyze 13 analytes in fresh tea leaves (with the treatment of dormancy, light qualities, exogenous hormones and infestation of pests), highlighting its sufficient capability for rapid analysis of multiclass phytohormones in agriculture field.

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