Transcriptomic Analysis of Mating Responses in Bemisia tabaci MED Females

文献类型： 外文期刊

第一作者： Huo, Zhijia

作者： Huo, Zhijia;Pang, Baoping;Liu, Yating;Yang, Jinjian;Xie, Wen;Wang, Shaoli;Wu, Qingjun;Zhang, Youjun;Zhou, Xuguo

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关键词： Bemisia tabaci MED; transcriptome; mating responses; DEG; female

期刊名称：INSECTS （ 影响因子：2.769； 五年影响因子：3.046 ）

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年卷期： 2020 年 11 卷 5 期

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收录情况： SCI

摘要： Mating triggers substantial changes in gene expression and leads to subsequent physiological and behavioral modifications. However, postmating transcriptomic changes responding to mating have not yet been fully understood. Here, we carried out RNA sequencing (RNAseq) analysis in the sweet potato whitefly, Bemisia tabaci MED, to identify genes in females in response to mating. We compared mRNA expression in virgin and mated females at 24 h. As a result, 434 differentially expressed gene transcripts (DEGs) were identified between the mated and unmated groups, including 331 up- and 103 down-regulated. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that many of these DEGs encode binding-related proteins and genes associated with longevity. An RT-qPCR validation study was consistent with our transcriptomic analysis (14/15). Specifically, expression of P450s (Cyp18a1 and Cyp4g68), ubiquitin-protein ligases (UBR5 and RNF123), Hsps (Hsp68 and Hsf), carboxylase (ACC-2), facilitated trehalose transporters (Tret1-2), transcription factor (phtf), and serine-protein kinase (TLK2) were significantly elevated in mated females throughout seven assay days. These combined results offer a glimpe of postmating molecular modifications to facilitate reproduction in B. tabaci females.

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