Targeted mutagenesis inArabidopsis thalianausing CRISPR-Cas12b/C2c1

文献类型： 外文期刊

第一作者： Wu, Fan

作者： Wu, Fan;Qiao, Xinyu;Zhao, Yafei;Zhang, Ziyi;Gao, Yifan;Shi, Lingfeng;Du, Haokun;Wang, Lulu;Kong, Dejing;Zhang, Ya-Jie;Liu, Langyu;Zhang, Yu;Wang, Quan;Kong, Dejing

作者机构：

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期： 2020 年 62 卷 11 期

页码：

收录情况： SCI

摘要： Cas12b/C2c1 is a newly identified class 2 CRISPR endonuclease that was recently engineered for targeted genome editing in mammals and rice. To explore the potential applications of the CRISPR-Cas12b system in the dicotArabidopsis thaliana, we selected BvCas12b and BhCas12b v4 for analysis. We successfully used both endonucleases to induce mutations, perform multiplex genome editing, and create large deletions at multiple loci. No significant mutations were detected at potential off-target sites. Analysis of the insertion/deletion frequencies and patterns of mutants generated via targeted gene mutagenesis highlighted the potential utility of CRISPR-Cas12b systems for genome editing inArabidopsis.

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