SH1-dependent maize seed development and starch synthesis via modulating carbohydrate flow and osmotic potential balance

文献类型： 外文期刊

第一作者： Zhang, Ke

作者： Zhang, Ke;Guo, Li;Liu, Baiyu;Li, Wendi;Zhang, Kewei;Li, Kunpeng;Cheng, Wen;Ding, Zhaohua;Wang, Fei;Xu, Changzheng;Zhao, Xiangyu

作者机构：

关键词： Glucose-1-phosphate; Seed development; SH1; Soluble sugars; Starch synthesis; Zea mays

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2020 年 20 卷 1 期

页码：

收录情况： SCI

摘要： Background As the main form of photoassimilates transported from vegetative tissues to the reproductive organs, sucrose and its degradation products are crucial for cell fate determination and development of maize kernels. Despite the relevance of sucrose synthase SH1 (shrunken 1)-mediated release of hexoses for kernel development, the underlying physiological and molecular mechanisms are not yet well understood in maize (Zea mays). Results Here, we identified a new allelic mutant ofSH1generated by EMS mutagenesis, designated assh1*. The mutation ofSH1caused more than 90% loss of sucrose synthase activity insh1*endosperm, which resulted in a significant reduction in starch contents while a dramatic increase in soluble sugars. As a result, an extremely high osmolality in endosperm cells ofsh1*was generated, which caused kernel swelling and affected the seed development. Quantitative measurement of phosphorylated sugars showed that Glc-1-P in endosperm ofsh1*(17 mu g g(- 1)FW) was only 5.2% of that of wild-type (326 mu g g(- 1)FW). As a direct source of starch synthesis, the decrease of Glc-1-P may cause a significant reduction in carbohydrates that flow to starch synthesis, ultimately contributing to the defects in starch granule development and reduction of starch content. Conclusions Our results demonstrated that SH1-mediated sucrose degradation is critical for maize kernel development and starch synthesis by regulating the flow of carbohydrates and maintaining the balance of osmotic potential.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序