Phylogenetic Relationship of PlantMLOGenes and Transcriptional Response ofMLOGenes toRalstonia solanacearumin Tomato

文献类型： 外文期刊

第一作者： Shi, Jianlei

作者： Shi, Jianlei;Wu, Weiren;Shi, Jianlei;Zai, Wenshan;Xiong, Zili;Wan, Hongjian

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关键词： mildew resistance locus O (MLO); bioinformatics; gene duplication; phylogenetic relationship; homologous genes; Ralstonia solanacearum; gene expression

期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）

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年卷期： 2020 年 11 卷 5 期

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收录情况： SCI

摘要： As a broad-spectrum disease resistance factor,MLOis involved in a variety of biotic and abiotic stress responses in plants. To figure out the structural features, phylogenetic relationships, and expression patterns ofMLOgenes, we investigated the genome and transcriptome sequencing data of 28 plant species using bioinformatics tools. A total of 197MLOgenes were identified. They possessed 5-7 transmembrane domains, but only partially contained a calmodulin-binding domain. A total of 359 polymorphic sites and 142 haplotypes were found in 143 sequences, indicating the rich nucleotide diversity ofMLOgenes. TheMLOgenes were unevenly distributed on chromosomes or scaffolds and were mainly located at the ends, forming clusters (24.1% genes), tandem duplicates (5.7%), and segment duplicates (36.2%). TheMLOgenes could be classified into three groups by phylogenetic analysis. The angiosperm genes were mainly in subgroup IA,Selaginella moellendorffiigenes were in subgroup IA and IIIB,Physcomitrella patensgenes were in subgroup IB and IIIA, and almost all algae genes were in group II. About half of theMLOgenes had homologs within and across species. The Ka/Ks values were all less than 1, varying 0.01-0.78, suggesting that purifying selection had occurred inMLOgene evolution. In tomato, RNA-seq data indicated thatSlMLOgenes were highly expressed in roots, followed by flowers, buds, and leaves, and also regulated by different biotic stresses. qRT-PCR analysis revealed thatSlMLOgenes could respond to tomato bacterial wilt, withSlMLO1,SlMLO2,SlMLO4,andSlMLO6probably involved in the susceptibility response, whereasSlMLO14andSlMLO16being the opposite. These results lay a foundation for the isolation and application of related genes in plant disease resistance breeding.

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